Team:BYU Provo/Notebook/Cholera - Detection/Summerexp

From 2013.igem.org

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| colspan="3" | <font color="#333399" size="5" font face="Calibri">  
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: '''Cholera Detection July Notebook'''</font>
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: '''Cholera Detection July - August Notebook'''</font>
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<font size="5" font face="Calibri"> '''July 1 - July 7''' </font>
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<font size="5" font face="Calibri"> '''July 1 - July 14''' </font>
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<font size="3" font face="Calibri"> Add stuff here </font>
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<font size="3" font face="Calibri"> We added Cro to our construct with the hope that overproduction of Cro will lead to the lysis of lambda. </font>
<br>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Summerexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:DetectionSpring1.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:BYUK_Aug2.JPG|250px|center|link=]]
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| <font size="5" font face="Calibri"> ''July 8 - July 14'' </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/PR| Progress Report]] </font>
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| <font size="5" font face="Calibri"> '''July 15 - July 21''' </font>
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| <font size="5" font face="Calibri"> '''July 15 - July 28''' </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Summerexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:BYUCDSummer3.JPG|250px|center|link=]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring3.JPG|250px|center]]
 
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| <font size="5" font face="Calibri"> '''July 21 - July 28''' </font>
 
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<font size="3" font face="Calibri"> Add description! </font>
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Dailylog|Daily log]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Explist|Experiment Listing]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/PR| Progress Report]] </font>
 
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<font size="3" font face="Calibri"> Add description! </font>
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<font size="3" font face="Calibri"> We have been having difficulties with lambda consistently going lytic. To correct this, we have plated 32 colonies from our original lambda containing strains to select for colonies that consistently go lytic when we provide stress. We found 3 strains that go lytic when we add H2O2. We will use those strains for our future tests. </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Summerexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Explist|Experiment Listing]]
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<br>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:BYUK_July22_2.JPG|250px|center|link=]]
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| style="width: 20%; background-color: transparent;"| [[File:Spring4.JPG|250px|center]]
 
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| <font size="5" font face="Calibri"> '''August 5 - August 19''' </font>
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| <font size="5" font face="Calibri"> '''August 5 - August 18''' </font>
<br>  
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<font size="3" font face="Calibri"> Add description! </font>
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<font size="3" font face="Calibri"> This week was spent trying to determine if lambda is stressed enough during electroporation to go lytic. If that is the case, it would explain why we never see plaques after doing an electroporation.  </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/SummerExp/Period4/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Explist|Experiment Listing]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:Spring3.JPG|250px|center]]
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| style="width: 20%; background-color: transparent;"| [[File:Kelton.png|250px|center|link=]]
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| <font size="5" font face="Calibri"> '''July 21 - July 28''' </font>
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| <font size="5" font face="Calibri"> '''August 19 - August 31''' </font>
<br>  
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<font size="3" font face="Calibri"> Add description! </font>
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<font size="3" font face="Calibri"> We made a great discovery! If we plate TT9907 next to cholera, lysis by bacteriophage lambda is induced! This revelation ignited a new plan for the cholera detection team.  To our knowledge it has never been shown that Cholera triggers the lytic phase of bacteriophage lambda, but upon researching we did learn that quorum sensing molecules (AHLs) from many types of bacteria are sensed by E.Coli's SdiA receptor protein. An unknown pathway from SdiA excites lambda into lysis.  We hypothesize that SdiA detects the AHL emitted by Vibrio cholerae, and induction of lambda is a downstream result of SdiA stimulation.  Our goal is to demonstrate this, and to mutate SdiA such that it becomes specific only to Cholera's AHL. </font>
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<font color="#333399" size="4" font face="Calibri">
<font color="#333399" size="4" font face="Calibri">
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Summerexp/Period5/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Explist|Experiment Listing]]
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<br>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/PR| Progress Report]] </font>
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| style="width: 20%; background-color: transparent;"| [[File:BYUK.JPG|250px|center|link=]]
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{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 03:26, 23 September 2013


Cholera Detection July - August Notebook



Cholera Detection
March-April
May-June
July-August
September-October

July 1 - July 14


We added Cro to our construct with the hope that overproduction of Cro will lead to the lysis of lambda.


Daily log


BYUK Aug2.JPG


July 15 - July 28


Add description!


Daily log


BYUCDSummer3.JPG


July 29 - August 4


We have been having difficulties with lambda consistently going lytic. To correct this, we have plated 32 colonies from our original lambda containing strains to select for colonies that consistently go lytic when we provide stress. We found 3 strains that go lytic when we add H2O2. We will use those strains for our future tests.


Daily log


BYUK July22 2.JPG


August 5 - August 18


This week was spent trying to determine if lambda is stressed enough during electroporation to go lytic. If that is the case, it would explain why we never see plaques after doing an electroporation.


Daily log


Kelton.png


August 19 - August 31


We made a great discovery! If we plate TT9907 next to cholera, lysis by bacteriophage lambda is induced! This revelation ignited a new plan for the cholera detection team. To our knowledge it has never been shown that Cholera triggers the lytic phase of bacteriophage lambda, but upon researching we did learn that quorum sensing molecules (AHLs) from many types of bacteria are sensed by E.Coli's SdiA receptor protein. An unknown pathway from SdiA excites lambda into lysis. We hypothesize that SdiA detects the AHL emitted by Vibrio cholerae, and induction of lambda is a downstream result of SdiA stimulation. Our goal is to demonstrate this, and to mutate SdiA such that it becomes specific only to Cholera's AHL.


Daily log


BYUK.JPG

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