Team:Chiba/Project/store

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Revision as of 12:46, 23 September 2013

iGEM-2013 Chiba

tolerance

background

    Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
    Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
    These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.

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 	<h2 id="store" style="background-color:#ff9933"><center>tolerance</center></h2>
+<h3>background</h3>
 	<p>&nbsp;&nbsp;&nbsp;&nbsp;Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>.
 	<br>&nbsp;&nbsp;&nbsp;&nbsp;Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.