Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.2W3110LiquidCulture

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'''V) Results'''
'''V) Results'''
: Growth of W3110 liquid cultures of bacteria to create glycerol stocks from.
: Growth of W3110 liquid cultures of bacteria to create glycerol stocks from.
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: The control tube showed no contamination.
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Latest revision as of 21:42, 17 June 2013


Phage Purification March - April Notebook: Experiments



Overview
March-April
May-June
July-August
September-October

4.2 W3110 Liquid Culture


I) Purpose

Grow bacteria for use in experiments.

II) Expected Outcome

Viable liquid culture of E. coli, W3110

III) Reagants Used

W3110 plated colonies
LB


IV) Actual Procedure

We put a colony from our W3110 plate of E.coli into 1mL LB broth and left in shaker at 37◦ overnight.
Repeated 4 times, leaving a total of 5 1 ml colonies.

V) Results

Growth of W3110 liquid cultures of bacteria to create glycerol stocks from.
The control tube showed no contamination.


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