Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.5PhageSpotTest

(Difference between revisions)

Latest revision as of 22:16, 17 June 2013

Phage Purification March - April Notebook: Experiments

4.5 Phage Spot Test

I) Purpose

Test for viable phage.

II) Expected Outcome

Plaques in the bacteria where phage can infect and kill.

III) Reagants Used

BL21

1x LB top agar

Phage: 14 T3

13 T5

10 T2

20OX171

40ØX174

IV) Actual Procedure

We plated together .5mL of BL21 with 1X LB TA.

We separated the plates into different segments and then spot tested the phage.

The plates were then put in a 37°C incubator.

V) Results

No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable.

@@ Line 44: / Line 44: @@
 : 1x LB top agar
 :Phage: 14 T3
-: : 13 T5
+:  13 T5
-: : 10 T2
+:  10 T2
-: : 20OX171
+:  20OX171
-: : 40ØX174
+:  40ØX174
 '''IV) Actual Procedure'''
 : We plated together .5mL of BL21 with 1X LB TA.
-We separated the plates into different segments and then spot tested the phage.
+: We separated the plates into different segments and then spot tested the phage.
-The plates were then put in a 37°C incubator
+: The plates were then put in a 37°C incubator.
 '''V) Results'''
 : No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable.