Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
(Difference between revisions)
(→Ligation) |
(→Ligation) |
||
Line 23: | Line 23: | ||
|- | |- | ||
|} | |} | ||
- | incubate 16°C | + | incubate 16 °C 1 hour |
</div> | </div> |
Revision as of 05:37, 24 September 2013
Aug 10
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
experiment | Pconst | 13.2 | spinach-DT | 2.1 | 3.5 |
experiment | Plac | 15.3 | spinach-DT | 2.1 | 3.5 |
experiment | Pconst | 13.2 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Plac | 15.3 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Ptet | 30.1 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Pconst | 13.2 | pT181 attenuator | 11.7 | 3.5 |
experiment | Plac | 15.3 | pT181 attenuator | 11.7 | 3.5 |
experiment | Ptet | 30.1 | RBS-lacZα-DT | 3.0 | 3.5 |
incubate 16 °C 1 hour