From 2013.igem.org
(Difference between revisions)
|
|
Line 1: |
Line 1: |
| ==Aug 10== | | ==Aug 10== |
- | ===Ligation===
| |
- | <div class="experiment">
| |
- | <span class="author">Kojima</span>
| |
- | {| class="wikitable"
| |
- | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
| |
- | |-
| |
- | |experiment||Pconst||13.2||spinach-DT||2.1||3.5
| |
- | |-
| |
- | |experiment||Plac||15.3||spinach-DT||2.1||3.5
| |
- | |-
| |
- | |experiment||Pconst||13.2||pT181 antisense (XbaI & PstI)||6.6||3.5
| |
- | |-
| |
- | |experiment||Plac||15.3||pT181 antisense (XbaI & PstI)||6.6||3.5
| |
- | |-
| |
- | |experiment||Ptet||30.1||pT181 antisense (XbaI & PstI)||6.6||3.5
| |
- | |-
| |
- | |experiment||Pconst||13.2||pT181 attenuator||11.7||3.5
| |
- | |-
| |
- | |experiment||Plac||15.3||pT181 attenuator||11.7||3.5
| |
- | |-
| |
- | |experiment||Ptet||30.1||RBS-lacZα-DT||3.0||3.5
| |
- | |-
| |
- | |}
| |
- | incubate 16 °C 1 hour
| |
- | </div>
| |
Revision as of 06:05, 24 September 2013
Aug 10