Template:Kyoto/Notebook/Aug 26

From 2013.igem.org

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|2||8/24 tetR aptamer 12_P||EcoRI&SpeI
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|2||8/24 tetR aptamer 12_P||EcoRI & SpeI
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|4||8/24 tetR aptamer 12_1R||EcoRI&SpeI
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|4||8/24 tetR aptamer 12_1R||EcoRI & SpeI
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|6||8/24 tetR aptamer 12_M||EcoRI&SpeI
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|6||8/24 tetR aptamer 12_M||EcoRI & SpeI
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[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Name||concentration[&micro;g/mL]||260/280||260/230
!Name||concentration[&micro;g/mL]||260/280||260/230
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|tetR aptamer 12_P(EcoRI&SpeI)||2.3||0.38||0.13
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|tetR aptamer 12_P (EcoRI & SpeI)||2.3||0.38||0.13
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|-
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|tetR aptamer 12_1R(EcoRI&SpeI)||12.0||0.63||1.19
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|tetR aptamer 12_1R (EcoRI & SpeI)||12.0||0.63||1.19
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|-
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|tetR aptamer 12_M(EcoRI&SpeI)||2.0||6.43||0.03
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|tetR aptamer 12_M (EcoRI & SpeI)||2.0||6.43||0.03
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===Ligation===  
===Ligation===  
<div class="experiment">
<div class="experiment">

Revision as of 06:08, 24 September 2013

Contents

Aug 26

Gel Extraction

Matsumoto and Ashida

LaneDNAEnzyme
1100bp ladder-
28/24 tetR aptamer 12_PEcoRI & SpeI
48/24 tetR aptamer 12_1REcoRI & SpeI
68/24 tetR aptamer 12_MEcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
tetR aptamer 12_P (EcoRI & SpeI)2.30.380.13
tetR aptamer 12_1R (EcoRI & SpeI)12.00.631.19
tetR aptamer 12_M (EcoRI & SpeI)2.06.430.03

Ligation

Ashida

stateVectorInserterLigation High ver.2
experiment8/21 DT5.08/26 tetR aptamer 12_1R2.03.5
experiment8/21 DT5.08/22 pT181 atteniator5.05.0
experiment8/21 DT5.08/22 pT181 antisense3.04.0
experiment8/21 DT5.08/22 Spinach3.04.0
experiment8/22 Plac4.08/22 pT181 attenuator5.04.5
experiment8/22 J231004.08/22 pT181 attenuator5.04.5
experiment8/22 Plac4.08/22 pT181 antisense8.06.0
experiment8/22 J231004.08/22 pT181 antisense8.06.0

Transformation

No name

NameSampleCompetent CellsTotalPlate
8/26 tetR aptamer 12_1R-DT1µL10µL11µLCP
8/26 pT181 attenuator-DT1µL10µL11µLCP
8/26 pT181 antisense-DT1µL10µL11µLCP
8/26 Spinach-DT1µL10µL11µLCP
8/26 Plac-pT181 attenuator1µL10µL11µLCP
8/26 Pcon-pT181 attenuator1µL10µL11µLAmp
8/26 Plac-pT181 antisense1µL10µL11µLCP
8/26 Pcon-pT181 antisense1µL10µL11µLAmp
Pλ-RBS-luxI-DT1µL10µL11µAmp
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