Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
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(→Waking Up Parts) |
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===LB Medium Plate=== | ===LB Medium Plate=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto & Tatsui</span> | ||
+ | {| class="wikitable" | ||
+ | !volume||200ml | ||
+ | |- | ||
+ | |Bacto T2ypton || 2g | ||
+ | |- | ||
+ | |Bacto yeast extract ||1g | ||
+ | |- | ||
+ | |NaCl||1g | ||
+ | |- | ||
+ | |0.4µ NaOHaq||50µL | ||
+ | |- | ||
+ | |Agar Pouder||2g | ||
+ | |} | ||
+ | </div> | ||
+ | |||
===Transformation=== | ===Transformation=== | ||
===Gel Making=== | ===Gel Making=== |
Revision as of 06:42, 24 September 2013
Contents |
Aug 10
Liquid Culture
Sample | medium |
---|---|
8/9 BBa-J23106 | LB(+amp) |
8/9 BBa-J23100-1 | LB(+amp) |
8/9 BBa-J23100-2 | LB(+amp) |
8/9 BBa-J44000-1 | LB(+amp) |
8/9 BBa-J44000-2 | LB(+amp) |
Waking Up Parts
Sample |
---|
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3 |
BBa-B0034(RBS)pSB1A2 |
BBa-I732020(RBS-lacZα-DTt)pSB1C3 |
BBa-P0412(RBS-lacI-DT)pSB1C3 |
BBa-R0011(Plac)pSB1C3 |
LB Medium Plate
volume | 200ml |
---|---|
Bacto T2ypton | 2g |
Bacto yeast extract | 1g |
NaCl | 1g |
0.4µ NaOHaq | 50µL |
Agar Pouder | 2g |