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Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
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| - | = GFP | + | = GFP under Arsenic Inducible Promoter = |
| - | To characterize | + | ''GFP production in relation with arsenite concentration'' |
| - | + | ||
| + | To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb | ||
Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD. | Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD. | ||
Revision as of 02:46, 25 September 2013
GFP under Arsenic Inducible Promoter
GFP production in relation with arsenite concentration
To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD.
