Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
(Difference between revisions)
(→Colony PCR) |
(→Electrophoresis) |
||
Line 40: | Line 40: | ||
===Electrophoresis=== | ===Electrophoresis=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Tatsui and Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !Lane||Sample | ||
+ | |- | ||
+ | | ||100bp ladder | ||
+ | |- | ||
+ | |4||8/9 BBa_J44000 -1 | ||
+ | |- | ||
+ | |4||8/9 BBa_J44000 -2 | ||
+ | |- | ||
+ | |6||NC | ||
+ | |} | ||
+ | [[File:igku_xxxxxx.xxx]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Lane||Sample | ||
+ | |- | ||
+ | | ||1kbp ladder | ||
+ | |- | ||
+ | |1||8/9 BBa_J23106 | ||
+ | |- | ||
+ | |2||8/9 BBa_J23100 -1 | ||
+ | |- | ||
+ | |3||8/9 BBa_J23100 -2 | ||
+ | |- | ||
+ | |4||NC | ||
+ | |} | ||
+ | [[File:igku_xxxxxx.xxx]]<br> | ||
+ | </div> | ||
+ | |||
===Liquid Culture=== | ===Liquid Culture=== | ||
<div class="experiment"> | <div class="experiment"> |
Revision as of 04:32, 25 September 2013
Contents |
Aug 10
Colony PCR
Sample |
---|
8/9 BBa_J44000 -(1) |
8/9 BBa_J44000 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 30 sec | 30 cycles |
Sample |
---|
8/9 BBa_J23106 |
8/9 BBa_J23100 -(1) |
8/9 BBa_J23100 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 1 min | 30 cycles |
Electrophoresis
Lane | Sample |
---|---|
100bp ladder | |
4 | 8/9 BBa_J44000 -1 |
4 | 8/9 BBa_J44000 -2 |
6 | NC |
Lane | Sample |
---|---|
1kbp ladder | |
1 | 8/9 BBa_J23106 |
2 | 8/9 BBa_J23100 -1 |
3 | 8/9 BBa_J23100 -2 |
4 | NC |
Liquid Culture
Sample | medium |
---|---|
8/9 BBa-J23106 | LB(+amp) |
8/9 BBa-J23100-1 | LB(+amp) |
8/9 BBa-J23100-2 | LB(+amp) |
8/9 BBa-J44000-1 | LB(+amp) |
8/9 BBa-J44000-2 | LB(+amp) |
Waking Up Parts
Sample |
---|
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3 |
BBa-B0034(RBS)pSB1A2 |
BBa-I732020(RBS-lacZα-DTt)pSB1C3 |
BBa-P0412(RBS-lacI-DT)pSB1C3 |
BBa-R0011(Plac)pSB1C3 |
LB Medium Plate(+Ampicillin)
volume | 200ml |
---|---|
Bacto T2ypton | 2g |
Bacto yeast extract | 1g |
NaCl | 1g |
0.4M NaOHaq | 500µL |
Agarose Pouder | 2g |
Ampicillin | 40µL |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
tetR | 3µL | 19µL | 22µL | Kan |
RBS | 3µL | 19µL | 22µL | Amp |
lacZα | 3µL | 19µL | 22µL | CP |
lacI | 3µL | 19µL | 22µL | CP |
Plac | 3µL | 19µL | 22µL | CP |