Template:Kyoto/Notebook/Aug 10

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|8/9 BBa-J44000-2||LB(+amp)  
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===Waking Up Parts===
 
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<div class="experiment">
 
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<span class="author">Nakamoto</span>
 
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!Sample
 
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|BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
 
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|BBa-B0034(RBS)pSB1A2
 
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|BBa-I732020(RBS-lacZ&alpha;-DTt)pSB1C3
 
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|BBa-P0412(RBS-lacI-DT)pSB1C3
 
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|BBa-R0011(Plac)pSB1C3
 
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Revision as of 04:36, 25 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles
Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec1 min30 cycles

Electrophoresis

Tatsui and Nakamoto

LaneSample
100bp ladder
48/9 BBa_J44000 -1
48/9 BBa_J44000 -2
6NC

File:Igku xxxxxx.xxx

LaneSample
1kbp ladder
18/9 BBa_J23106
28/9 BBa_J23100 -1
38/9 BBa_J23100 -2
4NC

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP

Gel Making

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