Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

(Difference between revisions)
(→Electrophoresis)
(→Electrophoresis)
Line 53: Line 53:
|6||NC
|6||NC
|}
|}
-
[[File:igku_xxxxxx.xxx]]<br>
+
[[File:Igku Aug10 Electrophoresis BBa 1.jpg]]<br>
{| class="wikitable"
{| class="wikitable"
!Lane||Sample
!Lane||Sample

Revision as of 13:28, 25 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles
Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec1 min30 cycles

Electrophoresis

Tatsui and Nakamoto

LaneSample
100bp ladder
48/9 BBa_J44000 -1
48/9 BBa_J44000 -2
6NC

Igku Aug10 Electrophoresis BBa 1.jpg

LaneSample
1kbp ladder
18/9 BBa_J23106
28/9 BBa_J23100 -1
38/9 BBa_J23100 -2
4NC

Igku Aug10electrophoresis1.jpg

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_10"