Template:Kyoto/Notebook/Sep 25

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(→Sep 25)
(→Colony PCR)
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|9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)||859
|9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)||859
|-
|-
-
|9/23 Pcon-PT181 attenuator-RBS-GFP-DT-(5~8)|1527
+
|9/23 Pcon-PT181 attenuator-RBS-GFP-DT-(5~8)||1527
|-
|-
|9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)||601
|9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)||601
|}
|}
 +
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94 °C||94 °C||55 °C||68 °C||--
 +
|-
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|2 min||30 s||30 s||1min5s||30 cycles
 +
|}
 +
</div>
 +
 +
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">no name</span>
 +
{| class="wikitable"
 +
!Lane||Sample||Enzyme1||Enzyme2
 +
|-
 +
|1||Pcon-PT181 attenuator-aptamer12-1R-DT-5||--||--
 +
|-
 +
|2||Pcon-PT181 attenuator-aptamer12-1R-DT-6||--||--
 +
|-
 +
|3||Pcon-PT181 attenuator-aptamer12-1R-DT-7||--||--
 +
|-
 +
|4||Pcon-PT181 attenuator-aptamer12-1R-DT-8||--||--
 +
|-
 +
|5||Pcon-PT181 attenuator-aptamer12-1R-DT-9||--||--
 +
|-
 +
|6||Pcon-PT181 attenuator-aptamer12-1R-DT-10||--||--
 +
|-
 +
|7||Pcon-PT181 attenuator-aptamer12-1R-DT-11||--||--
 +
|-
 +
|8||Pcon-PT181 attenuator-aptamer12-1R-DT-12||--||--
 +
|-
 +
|9||1kbp ladder||--||--
 +
|-
 +
|10||Pcon-PT181 attenuator-RBS-GFP-DT-5||--||--
 +
|-
 +
|11||Pcon-PT181 attenuator-RBS-GFP-DT-6||--||--
 +
|-
 +
|12||Pcon-PT181 attenuator-RBS-GFP-DT-7||--||--
 +
|-
 +
|13||Pcon-PT181 attenuator-RBS-GFP-DT-8||--||--
 +
|-
 +
|14||Pcon-PT181 attenuator-pSB1C3-5||--||--
 +
|-
 +
|15||Pcon-PT181 attenuator-pSB1C3-6||--||--
 +
|-
 +
|16||Pcon-PT181 attenuator-pSB1C3-7||--||--
 +
|-
 +
|17||Pcon-PT181 attenuator-pSB1C3-8||--||--
 +
|}
 +
[[File:xxxxx1.jpg]]<br>
 +
</div>
===Transformation===
===Transformation===

Revision as of 09:52, 26 September 2013

Contents

Sep 25

Colony PCR

Nakamoto

Samplebase pair
9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)859
9/23 Pcon-PT181 attenuator-RBS-GFP-DT-(5~8)1527
9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)601
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
2 min30 s30 s1min5s30 cycles


Electrophoresis

no name

LaneSampleEnzyme1Enzyme2
1Pcon-PT181 attenuator-aptamer12-1R-DT-5----
2Pcon-PT181 attenuator-aptamer12-1R-DT-6----
3Pcon-PT181 attenuator-aptamer12-1R-DT-7----
4Pcon-PT181 attenuator-aptamer12-1R-DT-8----
5Pcon-PT181 attenuator-aptamer12-1R-DT-9----
6Pcon-PT181 attenuator-aptamer12-1R-DT-10----
7Pcon-PT181 attenuator-aptamer12-1R-DT-11----
8Pcon-PT181 attenuator-aptamer12-1R-DT-12----
91kbp ladder----
10Pcon-PT181 attenuator-RBS-GFP-DT-5----
11Pcon-PT181 attenuator-RBS-GFP-DT-6----
12Pcon-PT181 attenuator-RBS-GFP-DT-7----
13Pcon-PT181 attenuator-RBS-GFP-DT-8----
14Pcon-PT181 attenuator-pSB1C3-5----
15Pcon-PT181 attenuator-pSB1C3-6----
16Pcon-PT181 attenuator-pSB1C3-7----
17Pcon-PT181 attenuator-pSB1C3-8----

File:Xxxxx1.jpg

Transformation

kojima and nakamoto

NameSampleCompetent CellsTotalPlate
Plac+tet aptamer12+Pcon tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-Spinach-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181antisense+DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator+tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon tetR-DT+Ptet+RBS-GFP-DT1µL10µL11µL-
Pcon tetR-DT+Pcon-GFP-DT+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-pT181attenuator-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-RBS-GFP-DT+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Pcon-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT1µL10µL11µL-

NameSampleCompetent CellsTotalPlate
aptamer12-DT(EcoRI+Xbal)+Pcon-pT181attenuator(EcoRI+Spel)2µL20µL22µL-
Ptet(Spel+Pstl)+RBS-GFP-DT(Xbal+Pstl))2µL20µL22µL-

Ligation

nakamoto

stateVectorInserterLigation High ver.2
experiment9/25 aptamer12-xx µLnamexx µLxx µL
NCnamexx µLnamexx µLxx µL

|experiment||name||xx µL||name||xx µL||xx µL |- |NC||name||xx µL||name||xx µL||xx µL

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