Team:XMU-China/wetlab journal

From 2013.igem.org

(Difference between revisions)
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id="i1s0i03" class="M1_exp_img"></div> </td> <td  
id="i1s0i03" class="M1_exp_img"></div> </td> <td  
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class="C1_td_text"> <textarea id="i1s0i01" class="C1_text" >8th  
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class="C1_td_text"> <textarea id="i1s0i01" class="C1_text" >8th August
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+
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August
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Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Bacteria:BL21(DE3)
Bacteria:BL21(DE3)
-
Condition: No flowing medium culture supply. Cells trap in the micro pools. Without  
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Condition: No flowing medium culture supply. Cells trap in the micro pools. Without antibiotic putting in.
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+
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antibiotic putting in.
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Total Obserbation Time:200mins
Total Obserbation Time:200mins
Analysis:  
Analysis:  
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At the beginning, the population of cells is small and the fluorescence degree is as the  
+
At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew as well as stronger fluorescence was detected. From then on, we observed weak oscillation of the fluorescence intensity.
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+
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same. Minutes later, more cells grew as well as stronger fluorescence was detected. From  
+
-
 
+
-
then on, we observed weak oscillation of the fluorescence intensity.
+
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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Bacteria:BL21(DE3)
Bacteria:BL21(DE3)
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic  
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic  
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putting in.
putting in.
Total Obserbation Time:140mins
Total Obserbation Time:140mins
Analysis:  
Analysis:  
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At the beginning, the population of cells is small and the fluorescence degree is as the  
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At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew but the population is still not large enough to observe convictive fluorescence intensity changing.
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+
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same. Minutes later, more cells grew but the population is still not large enough to observe  
+
-
 
+
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convictive fluorescence intensity changing.
+
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Bacteria:DH5a
Bacteria:DH5a
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Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic  
+
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic putting in.
-
 
+
-
putting in.
+
Total Obserbation Time:165mins
Total Obserbation Time:165mins
Analysis:  
Analysis:  
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At the beginning, the population of cells is small and the fluorescence degree is as the  
+
At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then on, the fluorescence degree kept in almost the same intensity.
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+
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same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then  
+
-
 
+
-
on, the fluorescence degree kept in almost the same intensity.
+
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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id="i1s3i03" class="M1_exp_img"></div> </td> <td  
id="i1s3i03" class="M1_exp_img"></div> </td> <td  
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class="C1_td_text"> <textarea id="i1s3i01" class="C1_text">31th  
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class="C1_td_text"> <textarea id="i1s3i01" class="C1_text">31th Auguest
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+
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Auguest
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Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Bacteria:DH5a
Bacteria:DH5a
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
-
antibiotic putting in.
+
Total Obserbation Time:80mins
Total Obserbation Time:80mins
Analysis:  
Analysis:  
At the beginning, the population of cell was large enough to get valid fluorescence degree.  
At the beginning, the population of cell was large enough to get valid fluorescence degree.  
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And from then on , we observed no fluorescence intensity changing.
And from then on , we observed no fluorescence intensity changing.
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
Line 309: Line 285:
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Bacteria:DH5a
Bacteria:DH5a
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
-
antibiotic putting in.
+
Total Obserbation Time:180mins
Total Obserbation Time:180mins
Analysis:  
Analysis:  
-
At the beginning, the population of cell was large enough to get valid fluorescence degree.  
+
At the beginning, the population of cell was large enough to get valid fluorescence degree. From then on ,there was  a weak decrease of the fluorescence degee.
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+
-
From then on ,there was  a weak decrease of the fluorescence degee.
+
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
-
antibiotic putting in.
+
Total Obserbation Time:515mins
Total Obserbation Time:515mins
Analysis:  
Analysis:  
-
From all the mircrofluid images, there is a tendency that the fluorescence degree change  
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From all the mircrofluid images, there is a tendency that the fluorescence degree change from weak to strong and then change from strong to weak.
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+
-
from weak to strong and then change from strong to weak.
+
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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Plasmid:sfGfp(coE1) aiia(pSB3T5)
Plasmid:sfGfp(coE1) aiia(pSB3T5)
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
-
antibiotic putting in.
+
Total Obserbation Time:590mins
Total Obserbation Time:590mins
Analysis:  
Analysis:  
There was a strong tendency that the fluorescence intensity came down, and it's the first  
There was a strong tendency that the fluorescence intensity came down, and it's the first  
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time that we detected the most obvious changing.
time that we detected the most obvious changing.
</textarea> <br> </td> </tr>
</textarea> <br> </td> </tr>
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id="i1s7i03" class="M1_exp_img"></div> </td> <td  
id="i1s7i03" class="M1_exp_img"></div> </td> <td  
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class="C1_td_text"> <textarea id="i1s7i01" class="C1_text">20th  
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class="C1_td_text"> <textarea id="i1s7i01" class="C1_text">20th September
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+
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September
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Plasmid:sfGfp(coE1) aiia(pSB3T5)
Plasmid:sfGfp(coE1) aiia(pSB3T5)
Bacteria: BL21(WT)
Bacteria: BL21(WT)
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
-
 
antibiotic putting in.
antibiotic putting in.
Total Obserbation Time:470mins
Total Obserbation Time:470mins
Line 518: Line 480:
Bacteria: BL21(WT)
Bacteria: BL21(WT)
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
-
 
antibiotic putting in.
antibiotic putting in.
Total Obserbation Time:?
Total Obserbation Time:?

Revision as of 14:29, 26 September 2013

导航栏

Microfluidic Experiment Record

2013/9/18 10:50:50--Chun Tang
2013/9/25 20:38:47


2013/9/18 10:51:01--Chun Tang
2013/9/25 20:42:38


2013/9/18 10:51:41--Chun Tang
2013/9/25 20:45:15


2013/9/18 10:52:00--Chun Tang
2013/9/25 20:46:04


2013/9/18 10:52:31--Chun Tang
2013/9/25 20:47:42


2013/9/18 10:53:02--Chun Tang
2013/9/25 20:50:35


2013/9/18 10:53:24--Chun Tang
2013/9/25 20:52:02


2013/9/25 21:10:34--Chun Tang
2013/9/25 21:11:06


2013/9/25 21:12:48--Chun Tang
2013/9/25 21:13:01


2013/9/25 21:15:00--Chun Tang
2013/9/25 21:15:47


2013/9/25 21:16:23


2013/9/25 21:16:51


Biobrick Transformation (6.24-6.30)

2013/9/26 20:16:17--Chun Tang
2013/9/26 20:16:39
2013/9/26 20:17:26


Plasmids Construction (7.1-7.28, 8.15-9.16)

2013/9/26 20:36:39--Chun Tang
2013/9/26 20:36:58
2013/9/26 20:37:10


2013/9/26 20:37:51--Chun Tang
2013/9/26 20:38:17
2013/9/26 20:38:31


2013/9/26 20:39:10


2013/9/26 20:40:10--Chun Tang
2013/9/26 20:40:27
2013/9/26 20:40:37


2013/9/26 20:42:21


2013/9/26 20:43:04--Chun Tang
2013/9/26 20:43:20
2013/9/26 20:43:33


2013/9/26 20:44:08


2013/9/26 20:44:56--Chun Tang
2013/9/26 20:45:14
2013/9/26 20:45:33


2013/9/26 20:46:05


2013/9/26 20:47:01--Chun Tang
2013/9/26 20:47:15
2013/9/26 20:47:24


2013/9/26 20:48:03


2013/9/26 20:48:37--Chun Tang
2013/9/26 20:48:50
2013/9/26 20:49:00


Transformation (7.29-9.20)

2013/9/26 20:49:56--Chun Tang
2013/9/26 20:50:04
2013/9/26 20:50:14


2013/9/26 20:51:15


2013/9/26 20:51:34--Chun Tang
2013/9/26 20:51:47
2013/9/26 20:52:17


2013/9/26 20:52:47--Chun Tang
2013/9/26 20:53:01
2013/9/26 20:53:09


2013/9/26 20:55:54--Chun Tang
2013/9/26 20:56:19

SDS-PAGE

2013/9/26 21:48:57--Tang Chun
2013/9/26 21:49:44
2013/9/26 21:50:08--Tang Chun
2013/9/26 21:51:07
2013/9/26 21:53:17--Tang Chun
2013/9/26 21:55:45
2013/9/26 22:02:08--Tang Chun
2013/9/26 22:03:34


2013/9/26 22:04:18--Tang Chun
2013/9/26 22:07:07


2013/9/26 22:07:33--Tang Chun
2013/9/26 22:08:04
2013/9/26 22:07:46


2013/9/26 22:08:31--Tang Chun
2013/9/26 22:08:47


2013/9/26 22:09:10--Tang Chun
2013/9/26 22:09:28


2013/9/26 22:09:40


2013/9/26 22:10:13--Tang Chun
2013/9/26 22:10:54


2013/9/26 22:11:28


2013/9/26 22:12:08--Tang Chun
2013/9/26 22:12:23


2013/9/26 22:12:32


2013/9/26 22:13:05--Tang Chun
2013/9/26 22:13:16



Retrieved from "http://2013.igem.org/Team:XMU-China/wetlab_journal"