Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
(Difference between revisions)
(→GFP production) |
FedeVignale (Talk | contribs) (→GFP under Arsenic Inducible Promoter) |
||
Line 8: | Line 8: | ||
Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD. | Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD. | ||
+ | [[File:Gfpinduccion.jpg|center|600px|]] | ||
+ | |||
+ | [[File:Gfp_ampliacion.jpg|center|600px|]] | ||
</div> | </div> |
Revision as of 15:21, 26 September 2013
GFP under Arsenic Inducible Promoter
GFP production in relation with arsenite concentration
To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD.