Team:Tianjin/Notebook

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<b>Figure 1.</b>AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT</div>
<b>Figure 1.</b>AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT</div>
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Revision as of 16:20, 26 September 2013

  • June,2013
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  • September,2013
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Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1.AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene




June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


July, 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July, 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


August, 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


August, 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene



August, 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene

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