Contents
- 1 June 7th, 2013
- 2 June 20th, 2013
- 3 June 26th, 2013
- 4 July, 5th, 2013
- 5 July 9th, 2013
- 6 July, 22nd, 2013
- 7 August, 1st, 2013
- 8 August, 10th, 2013
- 9 August 13th, 2013
- 10 August, 18th, 2013
- 11 August 23rd, 2013
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June 7th, 2013
Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.
Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT
Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT
Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT
June 20th, 2013
Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.
Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3
Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3
June 26th, 2013
Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.
Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3
July, 5th, 2013
Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.
Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3
July 9th, 2013
Translated E. coli BL21 with plasmids constructed.
July, 22nd, 2013
Acquired YbhGFSR through PCR and linked it with pSB3C5.
Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5
August, 1st, 2013
Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.
August, 10th, 2013
Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.
August 13th, 2013
Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.
August, 18th, 2013
Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.
August 23rd, 2013
Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.
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