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Index.Preparation of LB culture
From 2013.igem.org
(Difference between revisions)
(Created page with " <p>1>LB solid recipe(1L):<br> yeast extract 5g<br> agarose(100ml 1.5g) <br> sodium chloride 10g <br> peptone 10g. <>br and the liquid recipe, based on the mater...") |
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<br> sodium chloride 10g | <br> sodium chloride 10g | ||
<br> peptone 10g. | <br> peptone 10g. | ||
| - | <> | + | <br> and the liquid recipe, based on the materials given above, </p> |
<p>2>Calculate the reagents we need, preparation for 400ml and 900ml LB, and then weigh the relevant reagents and dissolve them.</p> | <p>2>Calculate the reagents we need, preparation for 400ml and 900ml LB, and then weigh the relevant reagents and dissolve them.</p> | ||
<p>3>Autoclave the culture to fully dissolve the reagents.</p> | <p>3>Autoclave the culture to fully dissolve the reagents.</p> | ||
Latest revision as of 18:12, 26 September 2013
1>LB solid recipe(1L):
yeast extract 5g
agarose(100ml 1.5g)
sodium chloride 10g
peptone 10g.
and the liquid recipe, based on the materials given above,
2>Calculate the reagents we need, preparation for 400ml and 900ml LB, and then weigh the relevant reagents and dissolve them.
3>Autoclave the culture to fully dissolve the reagents.
