Team:NJU China/Team

From 2013.igem.org

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<body>
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        <p>&nbsp;</p>
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        <div class="container">
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            <h2 class="ss-subtitle">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;TEAM</h2>
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<!--Start NavBar-->
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<ul id="nav">
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     <li><a href="https://2013.igem.org/Team:NJU_China">Home</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Team">Team</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Human Practice">Human Practice</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Modeling">Modeling</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Notebook</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a></li>
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    <li><a href="https://2013.igem.org/Team:NJU_China/Attributions">Attributions</a></li>
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                    <h2 id="March">TEAM</h2>
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                    </div>
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                    <div class="ss-right">
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                    <h2>NJU_China</h2>
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                    </div>
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                    </div>
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<div class="ss-left">
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<h3>
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<img width="300px"; height="400px" src="https://static.igem.org/mediawiki/2013/6/67/Gongfei.JPG">
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</h3>
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<div class="ss-right">
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<h3>
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<a>Gong Fei</a>
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<span>
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To strive,to seek, to find and never to give up.</br>
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Contact:gongfei1024@126.com</br>
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<div class="ss-right">
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<h3>
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<a>Dai Yimei</a>
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<span>
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Hi, my name is Dai Yimei and I am a senior at Nanjing University. I am majoring in life science and plan to pursue graduate study in developmental biology. I believe that science can work wonders. </br>
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Contact: yimei1112@163.com</br>
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-
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+
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+
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+
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-
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+
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+
}
}
 +
</style>
 +
        <link href='http://fonts.googleapis.com/css?family=Kelly+Slab' rel='stylesheet' type='text/css' />
 +
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<link rel="stylesheet" type="text/css" href="css/styleIE.css" />
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<script language="Javascript">
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</script>
 +
    </head>
 +
    <body>
 +
        <p>&nbsp;</p>
 +
        <div class="container">
-
/* pinterest like photo grid for social page*/
+
            <h2 class="ss-subtitle">Notebook</h2>
 +
<div id="ss-links" class="ss-links">
 +
<a href="#March">Mar</a>
 +
<a href="#April">Apr</a>
 +
<a href="#May">May</a>
 +
<a href="#June">Jun</a>
 +
<a href="#July">Jul</a>
 +
<a href="#August">Aug</a>
 +
                <a href="#September">Sep</a>
-
/*
+
</div>
-
body {
+
            <div id="ss-container" class="ss-container">
-
background: url(http://subtlepatterns.com/patterns/scribble_light.png) ;
+
                <div class="ss-row">
-
}
+
                    <div class="ss-left">
-
*/
+
                        <h2 id="March">March</h2>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h2>2013</h2>
 +
                    </div>
 +
                </div>
 +
                <div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
                        <a class="ss-circle ss-circle-1">Week 1 (25th-28th, March)</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
<a>Experiment training</a>
 +
<span>We learnt to culture the 293t cells.</span>
 +
<span>We learnt to extract plasmids and transfect it into 293t cells.</span>
 +
<span>We learnt to collect exosomes.</span>
 +
<span>We learnt to extract RNA from exosomes and cells.</span>
 +
<span>We learnt to do RT-PCR and qPCR.</span>                           
 +
                        </h3>
 +
                    </div>
 +
                </div>
-
#wrapper {
+
<div class="ss-row">
-
width: 90%;
+
                    <div class="ss-left">
-
max-width: 1100px;
+
                        <h2 id="April">April</h2>
-
min-width: 800px;
+
                    </div>
-
margin: 50px auto;
+
                    <div class="ss-right">
-
}
+
                        <h2>2013</h2>
-
 
+
                    </div>
-
#columns {
+
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-
-webkit-column-count: 3;
+
                <div class="ss-row ss-medium">
-
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+
                    <div class="ss-rihgt">
-
-webkit-column-fill: auto;
+
                        <a class="ss-circle ss-circle-2">Week2 (14th-18th, April)</a>
-
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+
                    </div>
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+
                </div>
-
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+
<div class="ss-row ss-small">
-
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+
                    <div class="ss-left">
-
column-gap: 15px;
+
                        <h3>
-
column-fill: auto;
+
<a>siRNA screening (Failed)</a>
-
}
+
<span>14th: We extracted plasmids and cultured the 293t cells.</span>
-
 
+
<span>15th: We transfected plasmids into 293t cells.</span>
-
.pin {
+
<span>16th: We collected cells and preserved it in Trizol.</span>
-
display: inline-block;
+
<span>17th: We extracted RNA, then did RT-PCR with the RNA.</span>
-
background: #FEFEFE;
+
<span>18th: We did qPCR with the cDNA we got on 17th April.</span>
-
border: 2px solid #FAFAFA;
+
<span>21th: We reexamined the concentration of RNA, and redid qPCR.</span>
-
box-shadow: 0 1px 2px rgba(34, 25, 25, 0.4);
+
                        </h3>
-
margin: 0 2px 15px;
+
                    </div>
-
-webkit-column-break-inside: avoid;
+
                    <div class="ss-row ss-medium">
-
-moz-column-break-inside: avoid;
+
                    <div class="ss-left">
-
column-break-inside: avoid;
+
                        <a class="ss-circle ss-circle-3">Week3 (27th, April – 1st, May)</a>
-
padding: 15px;
+
                    </div>
-
padding-bottom: 5px;
+
                </div>
-
background: -webkit-linear-gradient(45deg, #FFF, #F9F9F9);
+
<div class="ss-row ss-small">
-
opacity: 1;
+
                    <div class="ss-right">
-
+
                        <h3>
-
-webkit-transition: all .2s ease;
+
<a> siRNA screening (Failed)</a>
-
-moz-transition: all .2s ease;
+
<span>27th: We cultured the 293t cells in a 12-well plate.</span>
-
-o-transition: all .2s ease;
+
<span>28th: We transfected 293t cells.</span>
-
transition: all .2s ease;
+
<span>29th: We collected cells and extracted RNA from it.</span>
-
}
+
<span>30th: We did RT-qPCR with the RNA we extracted on 29th April.</span>
-
 
+
<span>1st: We did qPCR with the cDNA we got on 30th April.</span>
-
.pin img {
+
                        </h3>
-
width: 100%;
+
                    </div>
-
border-bottom: 1px solid #ccc;
+
                   
-
padding-bottom: 15px;
+
<div class="ss-row">
-
margin-bottom: 5px;
+
                    <div class="ss-left">
-
}
+
                        <h2 id="May">May</h2>
-
 
+
                    </div>
-
.pin p {
+
                    <div class="ss-right">
-
font: 12px/18px Arial, sans-serif;
+
                        <h2>2013</h2>
-
color: #333;
+
                    </div>
-
margin: 0;
+
                </div>
-
}
+
                <div class="ss-row ss-medium">
-
 
+
                    <div class="ss-left">
-
@media (min-width: 960px) {
+
                        <a class="ss-circle ss-circle-4">Week4 (8th-13th, May)</a>
-
#columns {
+
                    </div>
-
-webkit-column-count: 4;
+
                </div>
-
-moz-column-count: 4;
+
<div class="ss-row ss-small">
-
column-count: 4;
+
                    <div class="ss-left">
-
}
+
                        <h3>
-
}
+
<a> siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)</a>
-
 
+
<span>8th: We extracted plasmids of 3 kinds of siRNA.</span>
-
@media (min-width: 1100px) {
+
<span>9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.</span>
-
#columns {
+
<span>10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.</span>
-
-webkit-column-count: 5;
+
<span>11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.</span>
-
-moz-column-count: 5;
+
<span>12th: We extracted RNA from cells and exosomes.</span>
-
column-count: 5;
+
<span>13th: We did RT-PCR and qPCR.</span>
-
}
+
-
}
+
-
 
+
-
#columns:hover .pin:not(:hover) {
+
-
opacity: 0.4;
+
-
}
+
-
</STYLE>  
+
-
 
+
-
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:400,600' rel='stylesheet' type='text/css'>
+
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-15">Scrollbar Visibility with jScrollPane</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-16">Multi-level Photo Map</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>September 27, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Multi-level Photo Map</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-large">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-17">Responsive Image Gallery with Thumbnail Carousel</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>September 20, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Responsive Image Gallery with Thumbnail Carousel</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-small">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>September 12, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Elastislide - A Responsive jQuery Carousel Plugin</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-18">Elastislide - A Responsive jQuery Carousel Plugin</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-19">Slicebox - A fresh 3D image slider with graceful fallback </a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>September 5, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Slicebox - A fresh 3D image slider with graceful fallback </a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row">
 +
                    <div class="ss-left">
 +
                        <h2 id="august">August</h2>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h2>2013</h2>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-large">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>August 30, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Automatic Image Montage with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-20">Automatic Image Montage with jQuery</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-21">Image Zoom Tour with jQuery</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>August 23, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Image Zoom Tour with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-small">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>August 16, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Circular Content Carousel with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-22">Circular Content Carousel with jQuery</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-large">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-23">Portfolio Image Navigation with jQuery</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>August 9, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Portfolio Image Navigation with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>August 4, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Expanding Fullscreen Grid Portfolio</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-24">Expanding Fullscreen Grid Portfolio</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row">
 +
                    <div class="ss-left">
 +
                        <h2 id="july">July</h2>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h2>2013</h2>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-small">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-25">Content Rotator with jQuery</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>July 29, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Content Rotator with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-large">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>July 22, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Vertical Sliding Accordion with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com" class="ss-circle ss-circle-26">Vertical Sliding Accordion with jQuery</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-medium">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com" class="ss-circle ss-circle-27">Animated Text and Icon Menu with jQuery</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>July 12, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Animated Text and Icon Menu with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-small">
 +
                    <div class="ss-left">
 +
<h3>
 +
                            <span>July 5, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Fullscreen Slideshow with HTML5 Audio and jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-30">Fullscreen Slideshow with HTML5 Audio and jQuery</a>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-large">
 +
                    <div class="ss-left">
 +
                        <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-28">Sliding Background Image Menu with jQuery</a>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h3>
 +
                            <span>July 3, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Sliding Background Image Menu with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row">
 +
                    <div class="ss-left">
 +
                        <h2 id="june">June</h2>
 +
                    </div>
 +
                    <div class="ss-right">
 +
                        <h2>2013</h2>
 +
                    </div>
 +
                </div>
 +
<div class="ss-row ss-small">
 +
                    <div class="ss-left">
 +
                      <h3>
 +
                            <span>June 9, 2013</span>
 +
                            <a href="http://sc.chinaz.com/">Grid Navigation Effects with jQuery</a>
 +
                        </h3>
 +
                    </div>
 +
                    <div class="ss-right">
 +
<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-29">Grid Navigation Effects with jQuery</a>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </div>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
<script language="Javascript">
<script language="Javascript">
 +
/*
 +
* jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
 +
*
 +
* Uses the built in easing capabilities added In jQuery 1.1
 +
* to offer multiple easing options
 +
*
 +
* TERMS OF USE - jQuery Easing
 +
*
 +
* Open source under the BSD License.
 +
*
 +
* Copyright © 2008 George McGinley Smith
 +
* All rights reserved.
 +
*
 +
* Redistribution and use in source and binary forms, with or without modification,
 +
* are permitted provided that the following conditions are met:
 +
*
 +
* Redistributions of source code must retain the above copyright notice, this list of
 +
* conditions and the following disclaimer.
 +
* Redistributions in binary form must reproduce the above copyright notice, this list
 +
* of conditions and the following disclaimer in the documentation and/or other materials
 +
* provided with the distribution.
 +
*
 +
* Neither the name of the author nor the names of contributors may be used to endorse
 +
* or promote products derived from this software without specific prior written permission.
 +
*
 +
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
 +
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
 +
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
 +
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
 +
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
 +
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
 +
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
 +
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
 +
* OF THE POSSIBILITY OF SUCH DAMAGE.
 +
*
 +
*/
// t: current time, b: begInnIng value, c: change In value, d: duration
// t: current time, b: begInnIng value, c: change In value, d: duration
Line 949: Line 1,062:
});
});
 +
/*
 +
*
 +
* TERMS OF USE - EASING EQUATIONS
 +
*
 +
* Open source under the BSD License.
 +
*
 +
* Copyright © 2001 Robert Penner
 +
* All rights reserved.
 +
*
 +
* Redistribution and use in source and binary forms, with or without modification,
 +
* are permitted provided that the following conditions are met:
 +
*
 +
* Redistributions of source code must retain the above copyright notice, this list of
 +
* conditions and the following disclaimer.
 +
* Redistributions in binary form must reproduce the above copyright notice, this list
 +
* of conditions and the following disclaimer in the documentation and/or other materials
 +
* provided with the distribution.
 +
*
 +
* Neither the name of the author nor the names of contributors may be used to endorse
 +
* or promote products derived from this software without specific prior written permission.
 +
*
 +
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
 +
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
 +
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
 +
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
 +
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
 +
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
 +
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
 +
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
 +
* OF THE POSSIBILITY OF SUCH DAMAGE.
 +
*
 +
*/       
</script>
</script>
<script type="text/javascript">
<script type="text/javascript">

Revision as of 21:04, 26 September 2013

<!DOCTYPE html>

 

Notebook

March

2013

Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.

April

2013

siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

August

2013

July

2013

June

2013