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Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
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''GFP production in relation with arsenite concentration'' | ''GFP production in relation with arsenite concentration'' | ||
| - | To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under | + | To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 200 and 1000 ppb.)Fluorescence was then measured with a fluorimeter at 555nm excitation peak and 55nm emission peak. All was normalized by the culture density measured by OD (600nm). |
| - | Fluorescence was measured with a fluorimeter at 555nm | + | |
[[File:Gfpinduccion.jpg|center|600px|]] | [[File:Gfpinduccion.jpg|center|600px|]] | ||
Revision as of 22:59, 26 September 2013
GFP under Arsenic Inducible Promoter
GFP production in relation with arsenite concentration
To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 200 and 1000 ppb.)Fluorescence was then measured with a fluorimeter at 555nm excitation peak and 55nm emission peak. All was normalized by the culture density measured by OD (600nm).
