Team:BYU Provo/Safety

From 2013.igem.org

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: [[Team:BYU_Provo/Safety#Organisms_Used|Organisms Used]]
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: [[Team:BYU_Provo/Safety#Approval|Approval]]
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: [[Team:BYU_Provo/Safety#Coding_Regions_Used|Coding Regions Used]]
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: [[Team:BYU_Provo/Safety#Overview|Overview]]
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: [[Team:BYU_Provo/Safety#Risks|Risks]]
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: [[Team:BYU_Provo/Safety#Safety_Form|Safety Form]]
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: [[Team:BYU_Provo/Safety#Safety_Precautions|Safety Precautions]]
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: [[Team:BYU_Provo/Safety#Biosafety_Form_Part_2|Biosafety Form 2]]
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: [[Team:BYU_Provo/Safety#Downloadable_Forms|Downloadable Forms]]
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__NOTOC__==Organisms Used ==
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__NOTOC__==Approval==
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<to be deleted>Use this page to answer the questions on the  [[Safety | safety page]].
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====Chassis Organisms====
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{| class="wikitable"
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| No || Species || Strain No/Name || Risk Group || Source Link || Disease Risk
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|-
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| 1 || Bacteriophage T4Do  || T4 || 1 ||  ||
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|-
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| 2 || Bacteriophage T7  || T7 || 1 || ?? other phage||
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|-
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| 3 || ''E. coli''  || B || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 4 || ''E. coli''  || BL21 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 5 || ''E. coli''  || TT9901 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 6 || ''E. coli''  || TT9907 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 7 || ''E. coli''  || TT25281 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 8 || ''E. coli'' K12  || W3110 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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| 9 || ''Vibrio Cholerae''  || ATCC14035 || 2 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Vibrio&species=Cholerae ''Vibrio Cholerae''] ||
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|}
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====Highest Risk Group Listed====
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Greater than 1. For more information, please refer to
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*  [[Team:BYU Provo/Safety#Biosafety_Form_Part_2|iGEM 2013 Biosafety Form Part 2]]
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<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
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</html>
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<br>
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==Coding Region Used==
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====Parts Used and Submitted to the Registry====
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{| class="wikitable"
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| No || Part No || Source for physical DNA|| Species origin || Risk group || Function/Description
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| 1 || BBa_K1195000 || T4 || 1 ||  ||
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|-
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| 2 || BBa_K1195001 || T7 || 1 || ?? other phage||
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| 3 || BBa_K1195002 || B || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 4 || BBa_K1195003 || BL21 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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| 5 || BBa_K1195004 || TT9901 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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| 6 || BBa_K1195005 || TT9907 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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| 7 || BBa_K1195006 || TT25281 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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|-
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| 8 || BBa_K1195007 || W3110 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
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| 9 || BBa_K1195008 || ATCC14035 || 2 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Vibrio&species=Cholerae ''Vibrio Cholerae''] ||
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|}
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For a more detailed description of the parts we submitted to the iGEM registry, please visit our [[Team:BYU_Provo/Parts|Parts Submitted to the Registry Page]].
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<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
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</html>
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<br>
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==Risks==
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=====Do the biological materials used in your lab work pose risks to the safety and health of team members or others working in the lab?=====
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Yes. ''Vibrio cholerae'' can pose a risk to team member safety if ingested, handled improperly, or improperly disposed of (causing accidental exposure).
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===== Do the biological materials used in your lab work pose risks to the safety and health of the general public, if released by design or by accident? =====
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There are no plans for release of anything harmful. The safety practices, including proper disposal, were designed after consultation with a ''V. cholerae'' and bacterial pathogen expert, [http://lifesciences.byu.edu/~rar5 Dr. Richard Robison], BYU.
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=====Do the biological materials used in your lab work pose risks to the environment, if released by design or by accident?=====
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There are no risks associated with the environment.
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=====Do the biological materials used in your lab work pose risks to security through malicious misuse by individuals, groups, or countries?=====
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We believe our safety practices in the lab prevent malicious misuse by individuals, groups, and countries.
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=====If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?=====
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There would be no known risks. Our chassis organism is a BSL 1 E. coli. Of course caution must be used in any case. The bacteriophages we used also must be tested before any human use. 
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<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
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</html>
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<br>
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==Safety Precautions==
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=====Does your project include any design features to address safety risks?=====
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Not yet.
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=====What safety training have you received (or plan to receive in the future)?=====
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All members of the team have received safety training from our instructors as well as an [http://training.byu.edu/ytrain online safety training] run by our university, BYU.
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=====Please provide a link to your institution biosafety guidelines.=====
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[http://lifesciences.byu.edu/safety/LabSafety/SafetyPolicies.aspx College of Life Sciences - Safety Policies]
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=====Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.=====
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We operate under the rules and guidelines as outlined on the [http://lifesciences.byu.edu/safety/Home.aspx BYU College of Life Sciences' Safety Page]. An IBC application must be filed for recombinant DNA. We have filed out this IBC report and our project has been approved the BYU Research Safety committee.
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=====Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.=====
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[http://www.cdc.gov/biosafety/publications/bmbl5/index.htm Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition]
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=====According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab?=====
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BioSafety 2
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=====What is the Risk Group of your chassis organism(s), as you stated in question 1? If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking.=====
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BioSafety 1
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Safety forms were approved on 9/20/13 by David Lloyd and Julie McNamara.
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<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
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<br>
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==Overview ==
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==Biosafety Form Part 2==
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Brigham Young University has set protocols aimed at ensuring student safety in the laboratory. Students are required to wear long pants and close-toed, waterproof shoes and follow safety precautions for working with chemicals and biological agents (protective clothing including gloves, etc.). They are prohibited from eating in laboratory rooms. The labs are kept locked when not in use and are subjected to random inspections to ensure that safety procedures are followed.
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All biological agents we are working with are BSL1 (E. coli and various phages) or BSL2 (''Vibrio cholera'', ATCC 14035). Dr. Julianne Grose, our instructor, has received BYU approval to work with BSL-2 reagents. All students involved have been trained to handle cholera safely. We have followed current safety measures for disposing of biological samples in biohazard waste as well as cleaning glassware and desktops with bleach and/or ethanol.
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<br>
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All BioBrick parts created and used are non-hazardous and have been contained to the lab.
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The Institutional Biosafety Committee (IBC) at Brigham Young University supervises the use of recombinant DNA and handles other biological safety and hazard issues in experiments. Such use might involve constructing and handling DNA molecules in organisms. The IBC must review and approve all proposed experiments in advance of actual work. The link to there website is found [http://orca.byu.edu/ibc/ here]. We have submitted the necessary forms to our IBC and have received approval for our work.
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==Downloadable Forms==
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==Safety Form==
[https://static.igem.org/mediawiki/2013/6/69/BYUSafetyForm1.pdf BYU Safety Form 1.pdf]
[https://static.igem.org/mediawiki/2013/6/69/BYUSafetyForm1.pdf BYU Safety Form 1.pdf]

Latest revision as of 00:29, 27 September 2013


Safety


Approval
Overview
Safety Form

Approval

Safety forms were approved on 9/20/13 by David Lloyd and Julie McNamara.

Overview

Brigham Young University has set protocols aimed at ensuring student safety in the laboratory. Students are required to wear long pants and close-toed, waterproof shoes and follow safety precautions for working with chemicals and biological agents (protective clothing including gloves, etc.). They are prohibited from eating in laboratory rooms. The labs are kept locked when not in use and are subjected to random inspections to ensure that safety procedures are followed.

All biological agents we are working with are BSL1 (E. coli and various phages) or BSL2 (Vibrio cholera, ATCC 14035). Dr. Julianne Grose, our instructor, has received BYU approval to work with BSL-2 reagents. All students involved have been trained to handle cholera safely. We have followed current safety measures for disposing of biological samples in biohazard waste as well as cleaning glassware and desktops with bleach and/or ethanol.

All BioBrick parts created and used are non-hazardous and have been contained to the lab.

The Institutional Biosafety Committee (IBC) at Brigham Young University supervises the use of recombinant DNA and handles other biological safety and hazard issues in experiments. Such use might involve constructing and handling DNA molecules in organisms. The IBC must review and approve all proposed experiments in advance of actual work. The link to there website is found [http://orca.byu.edu/ibc/ here]. We have submitted the necessary forms to our IBC and have received approval for our work.

Safety Form

BYU Safety Form 1.pdf

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