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Team:HokkaidoU Japan/Notebook/Promoter WildPromoter-I732020
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| - | <h2></h2> | + | <h2>WildPromoter-I732020</h2> |
<div class="workflow-container"> | <div class="workflow-container"> | ||
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<div class="step-tile"><h2>Start</h2></div> | <div class="step-tile"><h2>Start</h2></div> | ||
</li> | </li> | ||
| - | + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jun 7</time> </li> </ul> <div class="step-recipe"> J23112, J23103, J23117, J23106, J23111 from distribution kit<br> each of 1µL DNA to DH5α </div> </div> </div> </li> | |
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jun 8 and Fri Jun 21</time> </li> </ul> <div class="step-recipe"> J23112, J23103, J23117, J23106, J23111<br> I732020 from our stock </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jun 9 and Sat Jun 22</time> </li> </ul> <div class="step-recipe"> J23112, J23103, J23117, J23106, J23111, I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Jun 26</time> </li> </ul> <div class="step-recipe"> I732020<br> 100up, 200dn, KOD Plus Neo </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Jun 10, Sat Jun 22 and Wed Jun 26</time> </li> </ul> <div class="step-recipe"> Cut (J23112, J23103, J23117, J23106, J23111) with SpeI, PstI (using 10×H buffer)<br> Cut I732020 with XbaI, PstI (using 10×M buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Gel extraction</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Jun 11 and Mon Jun 24</time> </li> </ul> <div class="step-recipe"> J23112, J23103, J23117, J23106, J23111 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jun 30</time> </li> </ul> <div class="step-recipe"> J23112, J23103, J23117, J23106, J23111 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Jun 11, Mon Jun 24 and Sun Jun 30</time> </li> </ul> <div class="step-recipe"> Ligate (J23112, J23103, J23117, J23106, J23111) with I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Jun 11, Mon Jun 24 and Sun Jun 30</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020<br> each of 1µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR & Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jun 30</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Jul 1</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jul 5</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020<br> EX-f, PS-r, KOD FX NEO </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR purification</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jul 6</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jul 6</time> </li> </ul> <div class="step-recipe"> Cut K1084007 on pSB1C3 with EcoRI, PstI (using 10×H buffer)<br> Cut (J23112, J23103, J23117, J23106, J23111)-I732020 with EcoRI, PstI (using 10×H buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phophatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jul 7</time> </li> </ul> <div class="step-recipe"> pSB1C3 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jul 7</time> </li> </ul> <div class="step-recipe"> Ligate pSB1C3 with (J23112, J23103, J23117, J23106, J23111)-I732020 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jul 7</time> </li> </ul> <div class="step-recipe"> (J23112, J23103, J23117, J23106, J23111)-I732020 </div> </div> </div> </li> | ||
<li class="step complete"> | <li class="step complete"> | ||
<div class="step-tile"><h2>Complete!</h2></div> | <div class="step-tile"><h2>Complete!</h2></div> | ||
Revision as of 06:59, 27 September 2013
Maestro E.coli
Notebook
WildPromoter-I732020
-
Start
-
Transformation
J23112, J23103, J23117, J23106, J23111 from distribution kit
each of 1µL DNA to DH5α -
Culture
J23112, J23103, J23117, J23106, J23111
I732020 from our stock -
Mini-prep
J23112, J23103, J23117, J23106, J23111, I732020 -
PCR
I732020
100up, 200dn, KOD Plus Neo -
Digestion
Cut (J23112, J23103, J23117, J23106, J23111) with SpeI, PstI (using 10×H buffer)
Cut I732020 with XbaI, PstI (using 10×M buffer) -
Gel extraction
J23112, J23103, J23117, J23106, J23111 -
Antarctic phosphatase
J23112, J23103, J23117, J23106, J23111 -
Ligation
Ligate (J23112, J23103, J23117, J23106, J23111) with I732020 -
Transformation
(J23112, J23103, J23117, J23106, J23111)-I732020
each of 1µL DNA to DH5α -
Colony PCR & Culture
(J23112, J23103, J23117, J23106, J23111)-I732020 -
Mini-prep
(J23112, J23103, J23117, J23106, J23111)-I732020 -
PCR
(J23112, J23103, J23117, J23106, J23111)-I732020
EX-f, PS-r, KOD FX NEO -
PCR purification
(J23112, J23103, J23117, J23106, J23111)-I732020 -
Digestion
Cut K1084007 on pSB1C3 with EcoRI, PstI (using 10×H buffer)
Cut (J23112, J23103, J23117, J23106, J23111)-I732020 with EcoRI, PstI (using 10×H buffer) -
Antarctic phophatase
pSB1C3 -
Ligation
Ligate pSB1C3 with (J23112, J23103, J23117, J23106, J23111)-I732020 -
Transformation
(J23112, J23103, J23117, J23106, J23111)-I732020 -
Complete!
