Team:BYU Provo/Notebook/LargePhage/Summerexp/Period3/Dailylog

From 2013.igem.org

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{{TeamBYUProvo}}
{{TeamBYUProvo}}
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==July==
 
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==August==
 
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===8/2/13===
 
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Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We too the biggest plaques and put them in a mL of LB broth in an eppendorf.
 
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We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program imageJ to our best use.
 
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[[File:August10.jpg|400px]]
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<br>
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===8/5/13===
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{| width="100%"
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Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient.
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| colspan="3" | <font color="#333399" size="5" font face="Calibri">
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===8/7/13===
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: '''Large Phage July - August Notebook: August 1 - August 16 Daily Log'''</font>
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Today we picked from small plaques and diluted them in 1 mL of LB.  We didn't have grown bacteria so we just put the tubes in the fridge.
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We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band.
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<br>
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<br>
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===8/9/13===
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|- valign="top"
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Results from 8/7/13 plaque test:
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| style="width: 18%; background-color: transparent;"|
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[[File:Aug7-1.jpg|450px]]
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[[File:Aug7-2.jpg|450px]]
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<font color="#333399" size="3" font face="Calibri">
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===8/12/13===
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<font size = "4">
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===8/14/13===
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: <u> '''Large Phage''' </u> </font>
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===8/16/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]]
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===8/19/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Springexp|May-June]]
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===8/21/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Summerexp|July-August]]
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===8/23/13===
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: [[Team:BYU Provo/Notebook/LargePhage/Fallexp|September-October]]
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===8/26/13===
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</font>
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===8/28/13===
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| style="width: 82%; background-color: transparent;"|
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===8/30/13===
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<font face="Calibri" size="3">
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==September==
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<font size="4"> '''8/2/13''' </font>
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===9/2/13===
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===9/4/13===
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Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We took the biggest plaques and put them in a mL of LB broth in an eppendorf.
 +
We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program image to our best use.
-
===9/6/13===
 
-
===9/9/13===
+
[[File:August10.jpg|400px]]
 +
 
 +
<br>
 +
 
 +
<font size="4"> '''8/5/13''' </font>
 +
 
 +
Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient.
 +
 
 +
<br>
 +
 
 +
<font size="4"> '''8/7/13''' </font>
 +
 
 +
Today we picked from small plaques and diluted them in 1 mL of LB.  We didn't have grown bacteria so we just put the tubes in the fridge.
 +
 
 +
We also drafted our project description as a class and posted it to our web site.
 +
 
 +
<br>
 +
 
 +
<font size="4"> '''8/9/13''' </font>
 +
 
 +
We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band.
 +
 
 +
Results from 8/7/13 plaque test:
 +
 
 +
 
 +
[[File:Aug7-1.jpg|450px]]
 +
 
 +
[[File:Aug7-2.jpg|450px]]
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===9/11/13===
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<br>
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===9/13/13===
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<font size="4"> '''8/12/13''' </font>
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===9/16/13===
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Today we gave the phage purification group another round of mutagenized phage to run through a CsCl gradient.  They will separate each band into fractions.
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===9/18/13===
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<br>
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===9/20/13===
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<font size="4"> '''8/14/13''' </font>
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===9/23/13===
 
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===9/25/13===
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[[File:Aug14-1.jpg|400px]]
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===9/27/13===
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[[File:Aug14-2.jpg|400px]]
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===9/30/13===
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<br>
{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 18:03, 27 September 2013


Large Phage July - August Notebook: August 1 - August 16 Daily Log



Large Phage
March-April
May-June
July-August
September-October

8/2/13

Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We took the biggest plaques and put them in a mL of LB broth in an eppendorf. We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program image to our best use.


August10.jpg


8/5/13

Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient.


8/7/13

Today we picked from small plaques and diluted them in 1 mL of LB. We didn't have grown bacteria so we just put the tubes in the fridge.

We also drafted our project description as a class and posted it to our web site.


8/9/13

We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band.

Results from 8/7/13 plaque test:


Aug7-1.jpg

Aug7-2.jpg


8/12/13

Today we gave the phage purification group another round of mutagenized phage to run through a CsCl gradient. They will separate each band into fractions.


8/14/13


Aug14-1.jpg

Aug14-2.jpg


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