Team:Cornell/project/wetlab/fungal toolkit
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<h5>Method</h5> | <h5>Method</h5> | ||
Protoplasting was first attempted on <i>Ganoderma lucidum</i>. Usually this species is protoplasted using lywallzyme and novozyme. However, these enzymes are exclusievly available in China and thus protplasting was attempted with driselease and glucanex. After several attempts it was concluded that the enzymes were unable to digest the cell wall without also killing the cell. In addition, the Ganoderma mycelium was hard to pellet when centrifuging and made the protoplasting procedure difficult. Thus, <i>Cochliobolus heterostrophus</i> was used instead. Protoplasting was sucessful using driselease and glucanex and then transformed via PEG solution. | Protoplasting was first attempted on <i>Ganoderma lucidum</i>. Usually this species is protoplasted using lywallzyme and novozyme. However, these enzymes are exclusievly available in China and thus protplasting was attempted with driselease and glucanex. After several attempts it was concluded that the enzymes were unable to digest the cell wall without also killing the cell. In addition, the Ganoderma mycelium was hard to pellet when centrifuging and made the protoplasting procedure difficult. Thus, <i>Cochliobolus heterostrophus</i> was used instead. Protoplasting was sucessful using driselease and glucanex and then transformed via PEG solution. | ||
+ | <h3>References</h3> | ||
+ | References here | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 18:44, 27 September 2013
Fungal Toolkit
Scroll over each icon to find out more about the functional requirements for our device!
Inlet
Water flows into our system, providing a sample to test water quality.![](https://static.igem.org/mediawiki/2013/7/7d/CornellCirclesPromoter.png)
![](https://static.igem.org/mediawiki/2012/9/91/TransBlock.png)
Filter
Prevents foreign microbes from entering and contaminating our reactor.![](https://static.igem.org/mediawiki/2013/2/21/CornellCirclesSelection.png)
Pumps
Move water and food to and from the reactor.![](https://static.igem.org/mediawiki/2013/a/a9/CornellCirclesCharacterization.png)
Piping and Calibration
Resist corrosion and set flow rates to the optimal level.![](https://static.igem.org/mediawiki/2013/8/89/CornellCirclesRecombination.png)
Reactors
House bacteria that produce electrical current in response to toxins.![](https://static.igem.org/mediawiki/2013/a/a4/CornellCirclesBiosafety.png)
![](https://static.igem.org/mediawiki/2013/9/9c/CornellCirclesDots.png)