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Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
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Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations. | Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations. | ||
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'''Methods''' | '''Methods''' | ||
Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). | Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). | ||
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'''Results''' | '''Results''' | ||
As shown in the graphics below GFP production increases with the arsenite concentration. | As shown in the graphics below GFP production increases with the arsenite concentration. | ||
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'''Conclusion''' | '''Conclusion''' | ||
The promoter is sensitive to different arsenite concentrations. | The promoter is sensitive to different arsenite concentrations. | ||
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[[File:Gfpinduccion.jpg|center|600px|]] | [[File:Gfpinduccion.jpg|center|600px|]] | ||
Revision as of 19:00, 27 September 2013
GFP under Arsenite Inducible Promoter
Objective
Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.
Methods
Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
Results
As shown in the graphics below GFP production increases with the arsenite concentration.
Conclusion
The promoter is sensitive to different arsenite concentrations.
