Team:SDU-Denmark/Tour42

SOPs

Standard operating procedures

“Ones more unto the breach, dear friends, once more” - Henry V

We used an arsenal of self-written Standard Operating Procedures (SOPs) to execute the vast amount of laboratory work. Each was written in collaboration with a person with experience in the field of the procedure and then approved by a second competent person. As such, it was strictly forbidden to change the SOPs without consulting the rest of the team, and any changes were then implemented in agreement. It was hereby secured that everyone in the lab were following the same procedures each time.

List of the different SOPs; Each name is a hyperlink connected to an online PDF of the SOP.
iGEM2013_SOP0001_v01_MM_ON_culture_of_E.coli
iGEM2013_SOP0002_v01_TK_Table_autoclave
iGEM2013_SOP0003_v01_MM_Bacterial_freezing_stock
iGEM2013_SOP0004_v01_Primer_Design
iGEM2013_SOP0005_v01_USER primer design
iGEM2013_SOP0006_v01_PCR protocol for USER cloning
iGEM2013_SOP0007_v01_excision and ligation of PCR product in USER cloning
iGEM2013_SOP0008_v01_preparing primers
iGEM2013_SOP0009_v01_TSB transformation
iGEM2013_SOP0010_v01_Phusion PCR
iGEM2013_SOP0011_v01_PCR with MyTaq
iGEM2013_SOP0012_v01_Restriction_Digest
iGEM2013_SOP0013_v01_Nano-drop
iGEM2013_SOP0014_v01_Gel purification
iGEM2013_SOP0015_v01_Ligation
iGEM2013_SOP0016_v01_Making LB and LA media
iGEM2013_SOP0017_v01_Fast_digest
iGEM2013_SOP0018_v01_Agar plates with Cm
iGEM2013_SOP0019_v01_Plasmid Miniprep
iGEM2013_SOP0020_v01_MM_Agarose_gel_DNA
iGEM2013_SOP0021_v01_TK_Colony_PCR_with_MyTaq
iGEM2013_SOP0022_v01_Speedy Vac
iGEM2013_SOP0023_v01_SDM_ PCR reactions for Site directed mutagenesis of Dxs from B. subtilis
iGEM2013_SOP0024_v01_Growth_experiment
iGEM2013_SOP0025_v01_Growth for RNA purification - IPTG inducable plasmid
iGEM2013_SOP0026_v01_Growth for RNA purification - ara inducible plasmid
iGEM2013_SOP0027_v01_RNA purification
iGEM2013_SOP0028_v01_Northen blotting
iGEM2013_SOP0029_v01_Kinating probe for northern blot