Team:Buenos Aires/ res rfpdeg

From 2013.igem.org

(Difference between revisions)
(GFP under Arsenite Inducible Promoter (Bba_K1106004))
Line 22: Line 22:
-
'''Conclusion'''
+
[[File:Gfpinduccion.jpg|center|600px|]]
-
The promoter is sensitive to different arsenite concentrations.
+
[[File:Gfpampliacion2.jpg|center|600px|]]
-
[[File:Gfpinduccion.jpg|center|600px|]]
+
'''Conclusion'''
-
 
+
-
[[File:Gfpampliacion2.jpg|center|600px|]]
+
 +
The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical concentrations according to the World Health Organisation.
</div>
</div>

Revision as of 21:32, 27 September 2013

GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004])



Objective

Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.


Methods

Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).


Results

As shown in the graphics below GFP production increases with the arsenite concentration.


Gfpinduccion.jpg
Gfpampliacion2.jpg


Conclusion

The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical concentrations according to the World Health Organisation.