Team:BYU Provo/Notebook/SmallPhage/Summerexp/9.13 Mutagen Concentration Test
From 2013.igem.org
(Difference between revisions)
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: - One that selects for large phage | : - One that selects for large phage | ||
- | * For their procedure, go here | + | * For their procedure, go here [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.16CsClGradient|9.16 CsCl Gradient]] |
4) Spot Test of Gradient Samples (9.18) | 4) Spot Test of Gradient Samples (9.18) | ||
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6) Plating to Check for Plaque Size 2 (9.23) | 6) Plating to Check for Plaque Size 2 (9.23) | ||
- | 7) Checking for Plaque Viability from Step 5 | + | * We redid the plating from step 5, but this time added a -3 dilution for some tubes (Small 11-13 and Large 3) because no plaques had formed at -4 for them. |
+ | |||
+ | 7) Checking for Plaque Viability from Step 5 (9.23) | ||
* We selected 4 plaques (looked bigger than normal) from the small phage plates and 6 plaques from the large phage plates (looked smaller than normal) from the plates in step 5. | * We selected 4 plaques (looked bigger than normal) from the small phage plates and 6 plaques from the large phage plates (looked smaller than normal) from the plates in step 5. | ||
- | * We picked each plaque with a pipet tip and placed them each in eppendorf | + | * We picked each plaque with a pipet tip and placed them each in eppendorf tubes with 100mL of LB. |
* We created a -1 and -2 dilution series for each plaque. | * We created a -1 and -2 dilution series for each plaque. | ||
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* Allowed them to incubate for 19 hours at 37 Celsius. | * Allowed them to incubate for 19 hours at 37 Celsius. | ||
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+ | 8) Checking for Plaque Viability 2 (9.25) | ||
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+ | * From the plates made in step 6, we selected more large and small plaques. We followed the same procedure as step 7 in the selection process, except we only made a -2 dilution. In total, we had 21 plaques from the small phage plates and 10 plaques from the large phage plates. | ||
Revision as of 21:34, 27 September 2013
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9.13 Mutagen Concentration Test - Ninth Protocol
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Overnight (The day before) (9.13)
2) Applying the mutagen (9.14)
3) Cesium Chloride Gradients
4) Spot Test of Gradient Samples (9.18)
5) Plating to Check for Plaque Size (9.20)
6) Plating to Check for Plaque Size 2 (9.23)
7) Checking for Plaque Viability from Step 5 (9.23)
8) Checking for Plaque Viability 2 (9.25)
2) Applying the mutagen
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