Team:Buenos Aires/ res rfpdeg

From 2013.igem.org

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(→GFP under Arsenic Inducible Promoter)
(→GFP under Arsenite Inducible Promoter (Bba_K1106004))
 
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= GFP under Arsenic Inducible Promoter =
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= GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) =
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''GFP production in relation with arsenite concentration''
 
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To characterize the response of the Arsenite sensible promoter several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb
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Fluorescence was measured with a fluorimeter at 555nm exitation wavelength and 55nm emission wavelegth. All these data was normalized by the culture density measured by OD.
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'''Objective'''
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Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concentrations.
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'''Methods'''
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Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
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'''Results'''
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As shown in the graphics below GFP production increases with the arsenite concentration. 
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[[File:Gfpinduccion.jpg|center|600px|]]
[[File:Gfpinduccion.jpg|center|600px|]]
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[[File:Gfp_ampliacion.jpg|center|600px|]]
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[[File:Gfpampliacion2.jpg|center|600px|]]
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'''Conclusion'''
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The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical arsenite concentrations according to the World Health Organisation.
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Latest revision as of 22:22, 27 September 2013

GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004])


Objective

Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concentrations.


Methods

Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).


Results

As shown in the graphics below GFP production increases with the arsenite concentration.


Gfpinduccion.jpg
Gfpampliacion2.jpg


Conclusion

The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical arsenite concentrations according to the World Health Organisation.

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