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Team:IIT Madras
From 2013.igem.org
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system (E.coli). The production of I3A will be validated by Crystal Violet biofilm assay. | system (E.coli). The production of I3A will be validated by Crystal Violet biofilm assay. | ||
<br><br>For the second part, we have identified a nine amino-acid peptide (Gb3 mimic) which has antiShiga toxin activity. To ensure the effective translocation of the peptide outside the cell, we plan | <br><br>For the second part, we have identified a nine amino-acid peptide (Gb3 mimic) which has antiShiga toxin activity. To ensure the effective translocation of the peptide outside the cell, we plan | ||
| - | to clone the Gb3 mimic downstream of an outer membrane signal peptide (ompF). The | + | to clone the Gb3 mimic downstream of an outer membrane signal peptide (ompF). The production of the Gb3 mimic will be confirmed by gel electrophoresis followed by mass spectrometry or reverse HPLC.</p> |
| - | production of the Gb3 mimic will be confirmed by gel electrophoresis followed by mass | + | |
| - | spectrometry or reverse HPLC.</p> | + | |
Revision as of 18:30, 30 June 2013
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Indian Institute of Technology Madras
iGEM 2013
iGEM and IIT Madras
IIT Madras has had a very successful history at previous iGEM competitions.
We started
participating in 2008 and in our very first iGEM, the team managed to secure a silver medal with
two prestigious awards for ‘Best Foundational Advance’ and ‘Best New BioBrick Part or
Device, Engineered.’
We backed our highly promising debut at the competition with another
silver medal at iGEM 2009.
The 2010 competition saw us rise to the zenith of our capabilities
and the team managed to bag IITM’s first gold medal at iGEM.
The legacy continued in iGEM 2011 as IITM received a ‘Safety Commendation’ and the award for the ‘Best New BioBrick Part, Natural’ in the Regional Jamboree and ultimately, another gold medal at the World
Championship held annually at MIT, USA.
Undergraduate students from the Department of Biotechnology (IITM), year after year, have
shown that they have the quality and the enthusiasm to compete and succeed at the highest level
and we really wish to continue the legacy of our institution this year as well.
About the Project
Shiga toxin is a deadly toxin secreted by Shigella dysenteriae and other shigatoxigenic bacteria.
These bacteria inhabit the rumen of cattle and produce a biofilm on the walls of the rumen which
is essential for the secretion of the toxin. According to statistics, more than 1 million people have
been killed by the lethal toxin and many more have been infected. There is no established cure
for this harmful toxin till date and this motivated us to come up with a feasible solution to this
global problem using synthetic biology.
Our idea mainly focuses on:
- Inhibiting biofilm production.
- Neutralizing the already produced toxin.
For the first part, we have identified a compound, indole-3-acetaldehyde (I3A), which is a potent
biofilm inhibitor. We plan to express the compound by engineering the I3A genes into our host
system (E.coli). The production of I3A will be validated by Crystal Violet biofilm assay.
For the second part, we have identified a nine amino-acid peptide (Gb3 mimic) which has antiShiga toxin activity. To ensure the effective translocation of the peptide outside the cell, we plan
