Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.10PhagePreparation

From 2013.igem.org

(Difference between revisions)

Latest revision as of 00:29, 28 September 2013

Phage Purification May - June Notebook: Experiments

Phage Purification

6.10 Phage Preparation

I) Purpose

Grow phage to practice purification procedures.

II) Expected Outcome

Viable stocks of both T4 and T7.

III) Reagants Used

W3110 and BL21 freezer stocks

LB

T4 and T7 phage stocks

IV) Actual Procedure

Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37^◦ C overnight.

Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.

Let both cultures sit overnight at 37^◦ C.

V) Results

We created phage cultures to practice the purification procedure.

@@ Line 10: / Line 10: @@
 |- valign="top"
-| style="width: 18%; background-color: transparent;"|
+| style="width: 20%; background-color: transparent;"|
 <font color="#333399" size="3" font face="Calibri">
-: [[Team:BYU_Provo/Small_Phage|Overview]]
+<font size = "4">
+: <u> '''Phage Purification''' </u> </font>
 : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
@@ Line 26: / Line 28: @@
 </font>
-| style="width: 82%; background-color: transparent;"|
+| style="width: 80%; background-color: transparent;"|
 <font face="Calibri" size="3">
-<font size="5"> '''6.10 Bacteria Cultures''' </font>
+<font size="5"> '''6.10 Phage Preparation''' </font>
 <br>
 '''I) Purpose'''
-: Grow bacteria for later use in preparation of phage.
+: Grow phage to practice purification procedures.
 '''II) Expected Outcome'''
-: Viable liquid cultures of both W3110 and BL21
+: Viable stocks of both T4 and T7.
 '''III) Reagants Used'''
-: W3110 and BL21 freezer stock
+: W3110 and BL21 freezer stocks
 : LB
+: T4 and T7 phage stocks
 '''IV) Actual Procedure'''
-: Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37<sup>◦</sup> C for 1 hour.
+: Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37<sup>◦</sup> C overnight.
-: Infect 4 mL of LB with 1 mL of liquid culture prepared in previous step.
+: Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.
-: Let both liquid cultures sit overnight at 37<sup>◦</sup> C.
+: Let both cultures sit overnight at 37<sup>◦</sup> C.
 '''V) Results'''
-: We were able to grow viable cultures of W3110 and BL21.
+: We created phage cultures to practice the purification procedure.
 </font>