From 2013.igem.org
(Difference between revisions)
Line 212:
Line 212: There is a difference between N and N@#A：
There is a difference between N and N@#A：
The band marked in green/red shows that E.coli cultured has a stronger expression of protein when added AHL?
The band marked in green/red shows that E.coli cultured has a stronger expression of protein when added AHL?
- </textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12"> <button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td> <td class="M1_exp_item_content"> <table id="i7s12i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br> <form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i04"> <input name="id" type="hidden" value="i7s12i0" form="i7s12i04"> <input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png"> <input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled=""> <div id="i7s12i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i01" class="C1_text"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i1">2013/9/28 08:47:11</td> <td class="M1_exp_item_content"> <table id="i7s12i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i10" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i4sds2.png"><br> <form id="i7s12i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i12" form="i7s12i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i14"> <input name="id" type="hidden" value="i7s12i1" form="i7s12i14"> <input name="oldgraph" type="hidden" id="i7s12i15" form="i7s12i14" value="t14i7s12i4sds2.png"> <input id="i7s12i16" type="submit" value="submit" form="i7s12i14" class="C1_button2" disabled=""> <div id="i7s12i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i11" class="C1_text"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i2">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i20" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i1sds3.png"><br> <form id="i7s12i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i22" form="i7s12i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i24"> <input name="id" type="hidden" value="i7s12i2" form="i7s12i24"> <input name="oldgraph" type="hidden" id="i7s12i25" form="i7s12i24" value="t14i7s12i1sds3.png"> <input id="i7s12i26" type="submit" value="submit" form="i7s12i24" class="C1_button2" disabled=""> <div id="i7s12i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i21" class="C1_text"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i3">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i30" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i2sds4.png"><br> <form id="i7s12i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i32" form="i7s12i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i34"> <input name="id" type="hidden" value="i7s12i3" form="i7s12i34"> <input name="oldgraph" type="hidden" id="i7s12i35" form="i7s12i34" value="t14i7s12i2sds4.png"> <input id="i7s12i36" type="submit" value="submit" form="i7s12i34" class="C1_button2" disabled=""> <div id="i7s12i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i31" class="C1_text"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i4">2013/9/28 08:44:28</td> <td class="M1_exp_item_content"> <table id="i7s12i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i40" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i3sds5.png"><br> <form id="i7s12i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i42" form="i7s12i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i44"> <input name="id" type="hidden" value="i7s12i4" form="i7s12i44"> <input name="oldgraph" type="hidden" id="i7s12i45" form="i7s12i44" value="t14i7s12i3sds5.png"> <input id="i7s12i46" type="submit" value="submit" form="i7s12i44" class="C1_button2" disabled=""> <div id="i7s12i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i41" class="C1_text">Description:
+ </textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12"> <button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td> <td class="M1_exp_item_content"> <table id="i7s12i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br> <form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i04"> <input name="id" type="hidden" value="i7s12i0" form="i7s12i04"> <input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png"> <input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled=""> <div id="i7s12i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i01" class="C1_text"> We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows.
+
+ Illustration:
+ A:BL21(WT)
+ B:BL21(WT) 1C3-sfgfp
+ C:BL21(WT) 1C3-sfgfp-aiiA
+ D:BL21(WT) 1C3-sfgfp-aiiA-ndh
+ E:3H-1C3-sfgfp-aiiA-ndh </textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i1">2013/9/28 08:47:11</td> <td class="M1_exp_item_content"> <table id="i7s12i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i10" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i4sds2.png"><br> <form id="i7s12i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i12" form="i7s12i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i14"> <input name="id" type="hidden" value="i7s12i1" form="i7s12i14"> <input name="oldgraph" type="hidden" id="i7s12i15" form="i7s12i14" value="t14i7s12i4sds2.png"> <input id="i7s12i16" type="submit" value="submit" form="i7s12i14" class="C1_button2" disabled=""> <div id="i7s12i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i11" class="C1_text">
We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows. +
+ Illustration:
+ A:BL21(WT)
+ B:BL21(WT) 1C3-sfgfp
+ C:BL21(WT) 1C3-sfgfp-aiiA
+ D:BL21(WT) 1C3-sfgfp-aiiA-ndh
+ E:3H-1C3-sfgfp-aiiA-ndh </textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i2">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i20" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i1sds3.png"><br> <form id="i7s12i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i22" form="i7s12i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i24"> <input name="id" type="hidden" value="i7s12i2" form="i7s12i24"> <input name="oldgraph" type="hidden" id="i7s12i25" form="i7s12i24" value="t14i7s12i1sds3.png"> <input id="i7s12i26" type="submit" value="submit" form="i7s12i24" class="C1_button2" disabled=""> <div id="i7s12i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i21" class="C1_text">
We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows. +
+ Illustration:
+ A:BL21(WT)
+ B:BL21(WT) 1C3-sfgfp
+ C:BL21(WT) 1C3-sfgfp-aiiA
+ D:BL21(WT) 1C3-sfgfp-aiiA-ndh
+ E:3H-1C3-sfgfp-aiiA-ndh </textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i3">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i30" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i2sds4.png"><br> <form id="i7s12i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i32" form="i7s12i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i34"> <input name="id" type="hidden" value="i7s12i3" form="i7s12i34"> <input name="oldgraph" type="hidden" id="i7s12i35" form="i7s12i34" value="t14i7s12i2sds4.png"> <input id="i7s12i36" type="submit" value="submit" form="i7s12i34" class="C1_button2" disabled=""> <div id="i7s12i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i31" class="C1_text">
We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows. +
+ Illustration:
+ A:BL21(WT)
+ B:BL21(WT) 1C3-sfgfp
+ C:BL21(WT) 1C3-sfgfp-aiiA
+ D:BL21(WT) 1C3-sfgfp-aiiA-ndh
+ E:3H-1C3-sfgfp-aiiA-ndh </textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i4">2013/9/28 08:44:28</td> <td class="M1_exp_item_content"> <table id="i7s12i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i40" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i3sds5.png"><br> <form id="i7s12i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i42" form="i7s12i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i44"> <input name="id" type="hidden" value="i7s12i4" form="i7s12i44"> <input name="oldgraph" type="hidden" id="i7s12i45" form="i7s12i44" value="t14i7s12i3sds5.png"> <input id="i7s12i46" type="submit" value="submit" form="i7s12i44" class="C1_button2" disabled=""> <div id="i7s12i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i41" class="C1_text">Description:
We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows.
We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows.
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