Team:Tianjin/Notebook

From 2013.igem.org

(Difference between revisions)
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});
});
</script>
</script>
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<div class="cont">
 
-
        <ul>
 
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            <li class="hmain" style="margin-top:20px;">
 
-
                    <a href="https://2013.igem.org/Team:Tianjin/Notebook">Overview</a>
 
-
                <ul>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Notebook/Experiment">Experiment Notebook</a>
 
-
                    </li>
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Notebook/Modeling">Modeling Notebook</a>
 
-
                    </li>                   
 
-
                    <li>
 
-
                        <a href="https://2013.igem.org/Team:Tianjin/Notebook/HumanPractice ">Human Practice Notebook</a>
 
-
                    </li>
 
-
                </ul>
 
-
            </li>
 
-
      </ul>
 
-
</div>
 
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<div class="cont">
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<div class="cont" style="margin-top:20px;">
         <ul>  
         <ul>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">June,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">June,2013</a>
                 <ul>  
                 <ul>  
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                     <li>
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                     <li>
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<style type="text/css">
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.table1 td{color:#d2d2d2;background-color: #fff; margin:1px;width:27px;height:27px;text-align:center;}
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.table1 td, .table1 th{font-size:14px;border:1px solid #fff;width:30px;text-align:center;}
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.table1 #alt{color:#fff;background-color: #4ccbfa; border:1px solid #fff;width:27px;height:27px;text-align:center;}
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.table1 th {font-size:14px;text-align:left;padding-top:5px;padding-bottom:5px;background-color:#d2d2d2;color:#fff;}
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.table1 th{font-size:14px;text-align:left;background-color:#d2d2d2;color:#fff; border:1px solid #fff;width:27px;height:27px;text-align:center;}
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.table1 tr td {color:#d2d2d2;background-color: #fff;}
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.table1 #alt a{color:#fff;background-color: #4ccbfa;width:27px;height:27px;text-align:center;margin:0px;padding:0px;border:none;}
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</style>
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<table class="table1">
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<table class="table1">
<tr>
<tr>
<th>Sun</th>
<th>Sun</th>
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<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td class="alt">1</td>
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<td>1</td>
</tr>
</tr>
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<td>3</td>
<td>3</td>
<td>4</td>
<td>4</td>
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<td class="alt">5</td>
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<td>5</td>
<td>6</td>
<td>6</td>
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<td>7</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-01">7</a></td>
<td>8</td>
<td>8</td>
</tr>
</tr>
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<td>12</td>
<td>12</td>
<td>13</td>
<td>13</td>
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<td class="alt">14</td>
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<td>14</td>
<td>15</td>
<td>15</td>
</tr>
</tr>
<tr>
<tr>
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<td class="alt">16</td>
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<td>16</td>
<td>17</td>
<td>17</td>
<td>18</td>
<td>18</td>
<td>19</td>
<td>19</td>
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<td>20</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-02">20</a></td>
<td>21</td>
<td>21</td>
<td>22</td>
<td>22</td>
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<td>24</td>
<td>24</td>
<td>25</td>
<td>25</td>
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<td class="alt">26</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">26</a></td>
<td>27</td>
<td>27</td>
<td>28</td>
<td>28</td>
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<tr>
<tr>
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<td class="alt">30</td>
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<td>30</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">July,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">July,2013</a>
                 <ul>  
                 <ul>  
                     <li>
                     <li>
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<td>3</td>
<td>3</td>
<td>4</td>
<td>4</td>
-
<td>5</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-04">5</a></td>
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<td class="alt">6</td>
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<td>6</td>
</tr>
</tr>
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<td>7</td>
<td>7</td>
<td>8</td>
<td>8</td>
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<td>9</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-05">9</a></td>
-
<td class="alt">10</td>
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<td>10</td>
<td>11</td>
<td>11</td>
<td>12</td>
<td>12</td>
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<td>17</td>
<td>17</td>
<td>18</td>
<td>18</td>
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<td class="alt">19</td>
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<td>19</td>
<td>20</td>
<td>20</td>
</tr>
</tr>
<tr>
<tr>
-
<td class="alt">21</td>
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<td>21</td>
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<td>22</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-06">22</a></td>
<td>23</td>
<td>23</td>
<td>24</td>
<td>24</td>
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<td>29</td>
<td>29</td>
<td>30</td>
<td>30</td>
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<td class="alt">31</td>
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<td>31</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">August,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">August,2013</a>
                 <ul>  
                 <ul>  
                     <li>
                     <li>
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<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>1</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-07">1</a></td>
<td>2</td>
<td>2</td>
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<td class="alt">3</td>
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<td>3</td>
</tr>
</tr>
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<td>5</td>
<td>5</td>
<td>6</td>
<td>6</td>
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<td class="alt">7</td>
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<td>7</td>
<td>8</td>
<td>8</td>
<td>9</td>
<td>9</td>
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<td>10</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-08">10</a></td>
</tr>
</tr>
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<td>11</td>
<td>11</td>
<td>12</td>
<td>12</td>
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<td>13</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-09">13</a></td>
<td>14</td>
<td>14</td>
<td>15</td>
<td>15</td>
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<td class="alt">16</td>
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<td>16</td>
<td>17</td>
<td>17</td>
</tr>
</tr>
<tr>
<tr>
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<td class="alt">18</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-10">18</a></td>
<td>19</td>
<td>19</td>
<td>20</td>
<td>20</td>
<td>21</td>
<td>21</td>
<td>22</td>
<td>22</td>
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<td>23</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">23</a></td>
<td>24</td>
<td>24</td>
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</tr>
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<td>26</td>
<td>26</td>
<td>27</td>
<td>27</td>
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<td class="alt">28</td>
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<td>28</td>
<td>29</td>
<td>29</td>
<td>30</td>
<td>30</td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">September,2013</a>
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                 <a href="https://2013.igem.org/wiki/index.php?title=Team:Tianjin/Notebook/HP">Human Practice Notebook</a>
-
                <ul>
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                    <li>
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<table class="table1">
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<tr>
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<th>Sun</th>
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<th>Mon</th>
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<th>Tue</th>
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<th>Wed</th>
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<th>Thu</th>
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<th>Fri</th>
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<th>Sat</th>
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</tr>
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-
 
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<tr>
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<td>1</td>
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<td>2</td>
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<td>3</td>
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<td class="alt">4</td>
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<td>5</td>
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<td>6</td>
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<td>7</td>
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</tr>
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<tr>
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<td>8</td>
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<td>9</td>
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<td>10</td>
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<td>11</td>
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<td>12</td>
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<td class="alt">13</td>
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<td>14</td>
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</tr>
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-
 
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<tr>
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<td class="alt">15</td>
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<td>16</td>
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<td>17</td>
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<td>18</td>
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<td>19</td>
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<td>20</td>
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<td>21</td>
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<tr>
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<td>22</td>
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<td>23</td>
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<td>24</td>
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<td class="alt">25</td>
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<td>26</td>
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<td>27</td>
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<td>28</td>
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</tr>
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-
 
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<tr>
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<td class="alt">29</td>
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<td>30</td>
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<td>&nbsp;</td>
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<td>&nbsp;</td>
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<td>&nbsp;</td>
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<td>&nbsp;</td>
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-
[[File:Tju-gel-1.1.1.jpg|center|400px|gel 1]]
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[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
<html>
<html>
<div style="text-align:center;">
<div style="text-align:center;">
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<b>Figure 1.</b>AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT</div>
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<b>Figure 1.</b>&nbsp;AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT</div>
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</div>
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</html>
 +
 
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[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
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<html>
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<div style="text-align:center;">
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<b>Figure 2.</b>&nbsp;AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT</div>
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</html>
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[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
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<html>
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<div style="text-align:center;">
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<b>Figure 3.</b>&nbsp;J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT</div>
<a name="anchor-n-02" id="anchor-n-02"></a>
<a name="anchor-n-02" id="anchor-n-02"></a>
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[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
<html>
<html>
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<div style="text-align:center;">
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<b>Figure 4.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3</div>
 +
</html>
 +
 +
 +
[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
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<html>
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<div style="text-align:center;">
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<b>Figure 5.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3</div>
 +
<a name="anchor-n-03" id="anchor-n-03"></a>
<a name="anchor-n-03" id="anchor-n-03"></a>
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[[File:Tju-notebook-1.3.jpg|center|450px|gene]]
[[File:Tju-notebook-1.3.jpg|center|450px|gene]]
 +
 +
 +
[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
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 +
<html>
 +
<div style="text-align:center;">
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<b>Figure 6.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3</div>
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<html>
<html>
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</html>
</html>
-
= July, 5th, 2013=
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= July 5th, 2013=
<html>
<html>
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[[File:Tju-notebook-1.4.jpg|center|330px|gene]]
[[File:Tju-notebook-1.4.jpg|center|330px|gene]]
 +
 +
 +
 +
[[File:Tju-gel-1.4.jpg|center|440px|gel 7]]
<html>
<html>
 +
<div style="text-align:center;">
 +
<b>Figure7.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3</div>
 +
<a name="anchor-n-05" id="anchor-n-05"></a>
<a name="anchor-n-05" id="anchor-n-05"></a>
<br/>
<br/>
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</html>
</html>
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= July, 22nd, 2013=
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= July 22nd, 2013=
<html>
<html>
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[[File:Tju-notebook-1.6.jpg|center|330px|gene 8]]
[[File:Tju-notebook-1.6.jpg|center|330px|gene 8]]
-
<html>
 
 +
 +
[[File:Tju-gel-1.6.jpg|center|350px|gel 8]]
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<html>
 +
<div style="text-align:center;">
 +
<b>Figure 8.</b>&nbsp;YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5</div>
<a name="anchor-n-07" id="anchor-n-07"></a>
<a name="anchor-n-07" id="anchor-n-07"></a>
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</html>
</html>
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= August, 1st, 2013=
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= August 1st, 2013=
<html>
<html>
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[[File:Tju-notebook-1.7.jpg|center|300px|gene]]
[[File:Tju-notebook-1.7.jpg|center|300px|gene]]
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 +
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[[File:Tju-gel-1.7.jpg|center|360px|gel 9]]
<html>
<html>
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<div style="text-align:center;">
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<b>Figure 9.</b>&nbsp;J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）</div>
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</html>
</html>
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= August, 10th, 2013=
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= August 10th, 2013=
<html>
<html>
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[[File:Tju-notebook-1.8.jpg|center|300px|gene 10]]
[[File:Tju-notebook-1.8.jpg|center|300px|gene 10]]
 +
 +
 +
 +
[[File:Tju-gel-1.8.jpg|center|350px|gel 10]]
<html>
<html>
 +
<div style="text-align:center;">
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<b>Figure 10.</b>&nbsp;J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5</div>
 +
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[[File:Tju-notebook-2.1.3.jpg|center|500px|gene]]
[[File:Tju-notebook-2.1.3.jpg|center|500px|gene]]
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 +
 +
[[File:Tju-gel-2.1.1.jpg|center|350px|gel 11]]
<html>
<html>
 +
<div style="text-align:center;">
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<b>Figure 11.</b>&nbsp;J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3</div>
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</html>
 +
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 +
[[File:Tju-gel-2.1.2.jpg|center|370px|gel 12]]
 +
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<html>
 +
<div style="text-align:center;">
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<b>Figure 12.</b>&nbsp;PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3</div>
 +
</html>
 +
 +
 +
[[File:Tju-gel-2.1.3.jpg|center|360px|gel 13]]
 +
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<html>
 +
<div style="text-align:center;">
 +
<b>Figure 13.</b>&nbsp;PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3</div>
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</html>
</html>
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= August, 18th, 2013=
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= August 18th, 2013=
<html>
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[[File:Tju-gel-2.2.1.jpg|center|500px|gel 14]]
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<b>Figure 14.</b>&nbsp;PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3</div>
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[[File:Tju-gel-2.2.2.jpg|center|450px|gel 15]]
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<b>Figure 15.</b>&nbsp;PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3</div>
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[[File:Tju-gel-2.3.jpg|center|350px|gel 16]]
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<b>Figure 16.</b>&nbsp;PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5</div>
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Latest revision as of 01:00, 28 September 2013

  • June,2013
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  • August,2013
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  • Human Practice Notebook

Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT


gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene


gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

July 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


gel 7

Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3

July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


gel 8

Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5

August 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


gel 9

Figure 9. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）

August 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


gel 10

Figure 10. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5

August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene


gel 11

Figure 11. J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3


gel 12

Figure 12. PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3


gel 13

Figure 13. PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3

August 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


gel 14

Figure 14. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3


gel 15

Figure 15. PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3

August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene


gel 16

Figure 16. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5

Retrieved from "http://2013.igem.org/Team:Tianjin/Notebook"