Team:Tianjin/Notebook

From 2013.igem.org

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         <ul>  
         <ul>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">June,2013</a>
+
                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">June,2013</a>
                 <ul>  
                 <ul>  
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.table1 #alt a{color:#fff;background-color: #4ccbfa;width:27px;height:27px;text-align:center;margin:0px;padding:0px;border:none;}
</style>
</style>
-
<div style="width:220px;">
 
-
        <div class="Calender0 ">Sun
 
-
        </div>
 
-
        <div class="Calender0">Mon
 
-
        </div>
 
-
        <div class="Calender0">Tue
 
-
        </div>
 
-
        <div class="Calender0">Wed
 
-
        </div>
 
-
        <div class="Calender0">Thu
 
-
        </div>
 
-
        <div class="Calender0">Fri
 
-
        </div>
 
-
        <div class="Calender0">Sat
 
-
        </div>
 
-
        <div class="Calender1">&nbsp;
 
-
        </div>
 
-
        <div class="Calender1">&nbsp;
 
-
        </div>
 
-
        <div class="Calender1">&nbsp;
 
-
        </div>
 
-
        <div class="Calender1">&nbsp;
 
-
        </div>
 
-
        <div class="Calender1">&nbsp;
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_6th.2C_2012">&nbsp;</a>
 
-
        </div>
 
-
        <div class="Calender1">1
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_8th.2C_2012">2</a>
 
-
        </div>
 
-
        <div class="Calender1">3
 
-
        </div>
 
-
        <div class="Calender1">4
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_11th.2C_2012">5</a>
 
-
        </div>
 
-
        <div class="Calender1">6
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_13th.2C_2012">7</a>
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_14th.2C_2012">8</a>
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_15th.2C_2012">9</a>
 
-
        </div>
 
-
        <div class="Calender1">10
 
-
        </div>
 
-
        <div class="Calender1">11
 
-
        </div>
 
-
        <div class="Calender1">12
 
-
        </div>
 
-
        <div class="Calender1">13
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_20th.2C_2012">14</a>
 
-
        </div>
 
-
        <div class="Calender1">15
 
-
        </div>
 
-
        <div class="Calender1">16
 
-
        </div>
 
-
        <div class="Calender1">17
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_24th.2C_2012">18</a>
 
-
        </div>
 
-
        <div class="Calender1">19
 
-
        </div>
 
-
        <div class="Calender1">20
 
-
        </div>
 
-
        <div class="Calender1">21
 
-
        </div>
 
-
        <div class="Calender1">22
 
-
        </div>
 
-
        <div class="Calender1">23
 
-
        </div>
 
-
        <div class="Calender2"><a href="#July_30th.2C_2012">24</a>
 
-
        </div>
 
-
        <div class="Calender1">25
 
-
        </div>
 
-
        <div class="Calender1">26
 
-
        </div>
 
-
        <div class="Calender1">27
 
-
        </div>
 
-
        <div class="Calender1">28
 
-
        </div>
 
-
        <div class="Calender1">29
 
-
        </div>
 
-
        <div class="Calender1">30
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        <div class="Calender1">
 
-
        </div>
 
-
        </div>
 
-
 
-
                     </li>
+
                      
-
                </ul>
+
<table class="table1">
-
            </li>
+
-
            <li class="hmain">
+
-
                <a href="#">July,2013</a>
+
-
                <ul>
+
-
                    <li>
+
-
             
+
-
<table class="table1">
+
<tr>
<tr>
<th>Sun</th>
<th>Sun</th>
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<tr>
<tr>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>1</td>
<td>1</td>
 +
</tr>
 +
 +
<tr>
<td>2</td>
<td>2</td>
<td>3</td>
<td>3</td>
<td>4</td>
<td>4</td>
<td>5</td>
<td>5</td>
-
<td class="alt">6</td>
+
<td>6</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-01">7</a></td>
 +
<td>8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>7</td>
 
-
<td>8</td>
 
<td>9</td>
<td>9</td>
-
<td class="alt">10</td>
+
<td>10</td>
<td>11</td>
<td>11</td>
<td>12</td>
<td>12</td>
<td>13</td>
<td>13</td>
 +
<td>14</td>
 +
<td>15</td>
</tr>
</tr>
<tr>
<tr>
-
<td>14</td>
 
-
<td>15</td>
 
<td>16</td>
<td>16</td>
<td>17</td>
<td>17</td>
<td>18</td>
<td>18</td>
-
<td class="alt">19</td>
+
<td>19</td>
-
<td>20</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-02">20</a></td>
 +
<td>21</td>
 +
<td>22</td>
</tr>
</tr>
<tr>
<tr>
-
<td class="alt">21</td>
 
-
<td>22</td>
 
<td>23</td>
<td>23</td>
<td>24</td>
<td>24</td>
<td>25</td>
<td>25</td>
-
<td>26</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">26</a></td>
<td>27</td>
<td>27</td>
 +
<td>28</td>
 +
<td>29</td>
</tr>
</tr>
<tr>
<tr>
-
<td>28</td>
 
-
<td>29</td>
 
<td>30</td>
<td>30</td>
-
<td class="alt">31</td>
+
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">August,2013</a>
+
                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">July,2013</a>
                 <ul>  
                 <ul>  
                     <li>
                     <li>
-
         
+
             
<table class="table1">
<table class="table1">
<tr>
<tr>
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<tr>
<tr>
-
<td>&nbsp;</td>
 
-
<td>&nbsp;</td>
 
-
<td>&nbsp;</td>
 
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>1</td>
<td>1</td>
<td>2</td>
<td>2</td>
-
<td class="alt">3</td>
+
<td>3</td>
-
</tr>
+
-
 
+
-
<tr>
+
<td>4</td>
<td>4</td>
-
<td>5</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-04">5</a></td>
<td>6</td>
<td>6</td>
-
<td class="alt">7</td>
 
-
<td>8</td>
 
-
<td>9</td>
 
-
<td>10</td>
 
</tr>
</tr>
<tr>
<tr>
 +
<td>7</td>
 +
<td>8</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-05">9</a></td>
 +
<td>10</td>
<td>11</td>
<td>11</td>
<td>12</td>
<td>12</td>
<td>13</td>
<td>13</td>
 +
</tr>
 +
 +
<tr>
<td>14</td>
<td>14</td>
<td>15</td>
<td>15</td>
-
<td class="alt">16</td>
+
<td>16</td>
<td>17</td>
<td>17</td>
 +
<td>18</td>
 +
<td>19</td>
 +
<td>20</td>
</tr>
</tr>
<tr>
<tr>
-
<td class="alt">18</td>
 
-
<td>19</td>
 
-
<td>20</td>
 
<td>21</td>
<td>21</td>
-
<td>22</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-06">22</a></td>
<td>23</td>
<td>23</td>
<td>24</td>
<td>24</td>
-
</tr>
 
-
 
-
<tr>
 
<td>25</td>
<td>25</td>
<td>26</td>
<td>26</td>
<td>27</td>
<td>27</td>
-
<td class="alt">28</td>
+
</tr>
 +
 
 +
<tr>
 +
<td>28</td>
<td>29</td>
<td>29</td>
<td>30</td>
<td>30</td>
<td>31</td>
<td>31</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
</tr>
</tr>
-
</table>  
+
 
 +
</table>
                     </li>  
                     </li>  
                 </ul>  
                 </ul>  
             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
-
                 <a href="#">September,2013</a>
+
                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">August,2013</a>
                 <ul>  
                 <ul>  
                     <li>
                     <li>
-
+
         
<table class="table1">
<table class="table1">
<tr>
<tr>
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<th>Sat</th>
<th>Sat</th>
</tr>
</tr>
-
 
-
 
<tr>
<tr>
-
<td>1</td>
+
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-07">1</a></td>
<td>2</td>
<td>2</td>
<td>3</td>
<td>3</td>
-
<td class="alt">4</td>
+
</tr>
 +
 
 +
<tr>
 +
<td>4</td>
<td>5</td>
<td>5</td>
<td>6</td>
<td>6</td>
<td>7</td>
<td>7</td>
 +
<td>8</td>
 +
<td>9</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-08">10</a></td>
</tr>
</tr>
<tr>
<tr>
-
<td>8</td>
 
-
<td>9</td>
 
-
<td>10</td>
 
<td>11</td>
<td>11</td>
<td>12</td>
<td>12</td>
-
<td class="alt">13</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-09">13</a></td>
<td>14</td>
<td>14</td>
 +
<td>15</td>
 +
<td>16</td>
 +
<td>17</td>
</tr>
</tr>
<tr>
<tr>
-
<td class="alt">15</td>
+
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-10">18</a></td>
-
<td>16</td>
+
-
<td>17</td>
+
-
<td>18</td>
+
<td>19</td>
<td>19</td>
<td>20</td>
<td>20</td>
<td>21</td>
<td>21</td>
 +
<td>22</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">23</a></td>
 +
<td>24</td>
</tr>
</tr>
<tr>
<tr>
-
<td>22</td>
+
<td>25</td>
-
<td>23</td>
+
-
<td>24</td>
+
-
<td class="alt">25</td>
+
<td>26</td>
<td>26</td>
<td>27</td>
<td>27</td>
<td>28</td>
<td>28</td>
-
</tr>
+
<td>29</td>
-
 
+
-
<tr>
+
-
<td class="alt">29</td>
+
<td>30</td>
<td>30</td>
-
<td>&nbsp;</td>
+
<td>31</td>
-
<td>&nbsp;</td>
+
-
<td>&nbsp;</td>
+
-
<td>&nbsp;</td>
+
-
<td>&nbsp;</td>
+
</tr>
</tr>
-
 
</table>  
</table>  
                     </li>  
                     </li>  
                 </ul>  
                 </ul>  
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/wiki/index.php?title=Team:Tianjin/Notebook/HP">Human Practice Notebook</a>
             </li>  
             </li>  
             <li>  
             <li>  
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</html>
-
= July, 5th, 2013=
+
= July 5th, 2013=
<html>
<html>
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</html>
-
= July, 22nd, 2013=
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= July 22nd, 2013=
<html>
<html>
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</html>
-
= August, 1st, 2013=
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= August 1st, 2013=
<html>
<html>
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</html>
-
= August, 10th, 2013=
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= August 10th, 2013=
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</html>
-
= August, 18th, 2013=
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= August 18th, 2013=
<html>
<html>

Latest revision as of 01:00, 28 September 2013

  • June,2013
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30            
  • July,2013
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30 31      
  • August,2013
    • Sun Mon Tue Wed Thu Fri Sat
              1 2 3
      4 5 6 7 8 9 10
      11 12 13 14 15 16 17
      18 19 20 21 22 23 24
      25 26 27 28 29 30 31
  • Human Practice Notebook

Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT


gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene


gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

July 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


gel 7

Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3

July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


gel 8

Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5

August 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


gel 9

Figure 9. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）

August 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


gel 10

Figure 10. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5

August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene


gel 11

Figure 11. J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3


gel 12

Figure 12. PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3


gel 13

Figure 13. PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3

August 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


gel 14

Figure 14. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3


gel 15

Figure 15. PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3

August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene


gel 16

Figure 16. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5

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