Team:Tianjin/Notebook

From 2013.igem.org

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                 <a href="#">June,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">June,2013</a>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-01">7</a></td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
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                 <a href="#">July,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">July,2013</a>
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<td>&nbsp;</td>
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             </li>  
             </li>  
             <li class="hmain">  
             <li class="hmain">  
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                 <a href="#">August,2013</a>
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                 <a href="https://2013.igem.org/Team:Tianjin/Notebook">August,2013</a>
                 <ul>  
                 <ul>  
                     <li>
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<td>&nbsp;</td>
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<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-07">1</a></td>
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             </li>  
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             <li class="hmain">  
             <li class="hmain">  
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                 <a href="#">September,2013</a>
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                 <a href="https://2013.igem.org/wiki/index.php?title=Team:Tianjin/Notebook/HP">Human Practice Notebook</a>
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Latest revision as of 01:00, 28 September 2013

  • June,2013
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30            
  • July,2013
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30 31      
  • August,2013
    • Sun Mon Tue Wed Thu Fri Sat
              1 2 3
      4 5 6 7 8 9 10
      11 12 13 14 15 16 17
      18 19 20 21 22 23 24
      25 26 27 28 29 30 31
  • Human Practice Notebook

Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT


gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene


gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

July 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


gel 7

Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3

July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


gel 8

Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5

August 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


gel 9

Figure 9. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）

August 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


gel 10

Figure 10. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5

August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene


gel 11

Figure 11. J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3


gel 12

Figure 12. PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3


gel 13

Figure 13. PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3

August 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


gel 14

Figure 14. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3


gel 15

Figure 15. PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3

August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene


gel 16

Figure 16. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5

Retrieved from "http://2013.igem.org/Team:Tianjin/Notebook"