From 2013.igem.org
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| | <div class="microfluidic"> | | <div class="microfluidic"> |
| | <h1>Biobrick Transformation (6.24-6.30)</h1> | | <h1>Biobrick Transformation (6.24-6.30)</h1> |
| - | <div class="M1_exp_stepInfo" title="Biobrick Transformation (6.24-6.30)" id="sb6s0" style=""> <button class="M1_exp_step_name_button" style=""><input value="Biobrick Transformation (6.24-6.30)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:16:17--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:16:39</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1" disabled="" style="background-color: white;">We rehydrated the registry DNA biobricks including 13-P1-12D, 13-P1-12F, 12-P2-21F, 13-P3-11H, and 12-P4-4A. Then we transformed them into BL21. After restriction analysis of the plasmids, we prepared glyoerol stocks for BL21 with 12D, 12F, and 21F.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/26 20:17:26</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10" src="http://trysomething-block.stor.sinaapp.com/ t14i6s0i11. png"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i11.png"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text" disabled="" style="background-color: white;">M:Marker DL500 | + | <div class="M1_exp_stepInfo" title="Biobrick Transformation (6.24-6.30)" id="sb6s0" style=""> <button class="M1_exp_step_name_button" style=""><input value="Biobrick Transformation (6.24-6.30)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:16:17--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:16:39</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1" disabled="" style="background-color: white;">We rehydrated the registry DNA biobricks including 13-P1-12D, 13-P1-12F, 12-P2-21F, 13-P3-11H, and 12-P4-4A. Then we transformed them into BL21. After restriction analysis of the plasmids, we prepared glyoerol stocks for BL21 with 12D, 12F, and 21F.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/26 20:17:26</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10" src="http://trysomething-block.stor.sinaapp.com/ t14i4s0i3%E5%9B%BE%E7%89%871. jpg"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i11.png"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text" disabled="" style="background-color: white;">M:Marker DL500 |
| | Lane1:12D plasmid | | Lane1:12D plasmid |
| | Lane2:12D plasmid, digestion of EcoRI,SpeI | | Lane2:12D plasmid, digestion of EcoRI,SpeI |
Revision as of 01:06, 28 September 2013
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