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Team:Chiba/Assay/oxidation
From 2013.igem.org
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| + | <h2>Experiment for measurement of oxidative state</h2> | ||
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<br>1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution. | <br>1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution. | ||
<br>2. Sterilized methylene blue solution using a filter. | <br>2. Sterilized methylene blue solution using a filter. | ||
<br>3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL. | <br>3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL. | ||
| - | + | <br>4. Precultured BL21and shuffle for 12 hours being cultured on methylene blue plates. | |
</p> | </p> | ||
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Latest revision as of 01:55, 28 September 2013
Experiment for measurement of oxidative state
1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution.
2. Sterilized methylene blue solution using a filter.
3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL.
4. Precultured BL21and shuffle for 12 hours being cultured on methylene blue plates.
