Team:AITM-Nepal/Part1
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+ | width:1050px; | ||
+ | -webkit-box-shadow:rgb(110,110,110) 8px 8px 8px; | ||
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+ | .maincontents | ||
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/* Logos css start */ | /* Logos css start */ | ||
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+ | .header | ||
+ | { | ||
+ | padding:20px; | ||
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#logo | #logo | ||
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/* Navbar css start */ | /* Navbar css start */ | ||
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- | + | background:-webkit-linear-gradient(Top,#666,#000); | |
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- | + | height:50px; | |
- | + | border:#000000 1px solid; | |
- | + | -webkit-box-shadow:rgb(110,110,110) 0px 8px 8px; | |
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+ | height:48px; | ||
+ | padding:10px; | ||
+ | } | ||
+ | #navigation li | ||
+ | { | ||
+ | display:inline; | ||
+ | list-style:none; | ||
+ | } | ||
- | + | ul li .navbar-option | |
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- | + | text-decoration:none; | |
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- | + | ul li .navbar-option:hover | |
- | { | + | { |
- | + | background:#0FF; | |
- | } | + | border-top:black 1px solid; |
+ | border-left:black 1px solid; | ||
+ | padding-left:9px; | ||
+ | padding-top:9px; | ||
+ | color:#000; | ||
+ | text-decoration:underline; | ||
+ | -webkit-transition: background 1s, color 1s; | ||
+ | } | ||
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+ | .sub_navigation | ||
+ | { | ||
+ | display:none; | ||
+ | list-style-type:none; | ||
+ | padding:10px; | ||
+ | position:absolute; | ||
+ | background:#000000; | ||
+ | color:#FFF; | ||
+ | -webkit-box-shadow:rgb(110,110,110) 0px 8px 8px; | ||
+ | } | ||
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+ | ul.sub_navigation li | ||
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+ | clear:both; | ||
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<!-- Javascript for student div show and hide Complete --> | <!-- Javascript for student div show and hide Complete --> | ||
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+ | <script> | ||
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+ | function togglemenu() | ||
+ | { | ||
+ | $('.sub_navigation').slideToggle(); | ||
+ | } | ||
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+ | </script> | ||
</head> | </head> | ||
+ | |||
+ | <div class="AllContent"> | ||
<!-- Logo Section Starts --> | <!-- Logo Section Starts --> | ||
- | <img id="logo" src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png"> | + | <div class="header"> |
- | <a href="https://2013.igem.org/Main_Page"><img id="logo2" src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png"></a> | + | <img id="logo" src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png"> |
+ | <img src="https://static.igem.org/mediawiki/2013/8/8e/1186269_700314813316746_1614415077_n.png" width="500px" height="140px" style="margi:auto;"> | ||
+ | <a href="https://2013.igem.org/Main_Page"><img id="logo2" src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png"></a> | ||
+ | </div> | ||
<!-- Logo Section Complete --> | <!-- Logo Section Complete --> | ||
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<!-- Navbar Section Start --> | <!-- Navbar Section Start --> | ||
- | + | <div class="navbar"> | |
+ | <div id="navigationboxholder"> | ||
+ | <ul id="navigation"> | ||
- | <li class=" | + | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal">Home</a></li> |
- | <li><a href="https://2013.igem.org/Team:AITM-Nepal/ | + | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Team">Team</a></li> |
- | + | <li><a onMouseOver="togglemenu();" href="https://2013.igem.org/Team:AITM-Nepal/Project" class="navbar-option">Project</a> | |
- | + | <ul class="sub_navigation"> | |
- | + | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Overview" class="navbar-option">Overview</a></li> | |
- | + | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Device_made" class="navbar-option">Device Made</a></li> | |
- | <li><a href="https://2013.igem.org/Team:AITM-Nepal/ | + | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part1" class="navbar-option">Part 1</a></li> |
- | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Safety | + | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part2" class="navbar-option">Part 2</a></li> |
- | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Human_practise | + | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part3" class="navbar-option">Part 3</a></li> |
- | <li><a href="https://2013.igem.org/Team:AITM-Nepal/Attributions | + | </ul></li> |
+ | <li><a class="navbar-option" href="https://igem.org/Team.cgi?year=2013&team_name=AITM-Nepal">Official Team Profile</a></li> | ||
+ | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Parts">Parts Submitted To Registry</a></li> | ||
+ | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Notebook">Notebook</a></li> | ||
+ | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a></li> | ||
+ | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Human_practise">Human Practice</a></li> | ||
+ | <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Attributions">Attributions</a></li> | ||
</ul> | </ul> | ||
+ | </div> | ||
+ | </div> | ||
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<!-- Main Section Starts --> | <!-- Main Section Starts --> | ||
- | + | <div class="maincontents"> | |
- | < | + | <h2> Part 1</h2> |
<p>Toll like receptor 8 transfection in HEK 293 cell line | <p>Toll like receptor 8 transfection in HEK 293 cell line | ||
Plasmid information : | Plasmid information : |
Latest revision as of 03:07, 28 September 2013
Part 1
Toll like receptor 8 transfection in HEK 293 cell line Plasmid information :
pcDNA3-TLR8-YFP | |
---|---|
Gene/insert name: | Toll-Like Receptor 8 |
Alt name: | TLR8 |
Insert size: | 3177 |
Species: | H. sapiens (human) |
GenBank ID: | NM_016610 |
Entrez Gene: | TLR8 (CD288, MGC119599, MGC119600) |
Fusion protein or tag: | YFP |
Terminal: | C terminal on backbone |
Vector backbone: | pcDNA3-YFP |
Vector type: | Mammalian Expression |
Backbone size w/o insert (bp): | 6100 |
Cloning site 5': | BamHI |
Site destroyed during cloning: | No |
Cloning site 3': | XhoI |
Site destroyed during cloning: | No |
5' sequencing primer: | T7 |
3' sequencing primer: | GTCTTGTAGTTGCCGTCGTC |
Bacterial resistance(s) | Ampicillin |
Growth strain(s) | DH5alpha | Growth temperature (℃): | 37 |
High or low copy: | High Copy |
Selectable markers: | Neomycin |