The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be expressed because the promoter should be induced by AHL.
The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be expressed because the promoter should be induced by AHL.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" > <button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td> <td class="M1_exp_item_content"> <table id="i7s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br> <form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i04"> <input name="id" type="hidden" value="i7s7i0" form="i7s7i04"> <input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png"> <input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;"> We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" > <button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td> <td class="M1_exp_item_content"> <table id="i7s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br> <form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i04"> <input name="id" type="hidden" value="i7s7i0" form="i7s7i04"> <input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png"> <input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;"> We were suggested that DH5α has a better viability and a higher protein expression. So there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
Data analysis:
Data analysis:
E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td> <td class="M1_exp_item_content"> <table id="i7s7i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br> <form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i14"> <input name="id" type="hidden" value="i7s7i1" form="i7s7i14"> <input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png"> <input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td> <td class="M1_exp_item_content"> <table id="i7s7i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br> <form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i14"> <input name="id" type="hidden" value="i7s7i1" form="i7s7i14"> <input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png"> <input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
Line 213:
Line 213:
There is a difference between N and N+A：
There is a difference between N and N+A：
The band marked in green/red shows that E.coli cultured has a stronger expression of protein when added AHL?
The band marked in green/red shows that E.coli cultured has a stronger expression of protein when added AHL?
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12"> <button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td> <td class="M1_exp_item_content"> <table id="i7s12i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br> <form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i04"> <input name="id" type="hidden" value="i7s12i0" form="i7s12i04"> <input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png"> <input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled=""> <div id="i7s12i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i01" class="C1_text"> We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp aiiA, sfgfp aiiA ndh. SDS-PAGE gel pictures are shown as follows.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12"> <button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td> <td class="M1_exp_item_content"> <table id="i7s12i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br> <form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i04"> <input name="id" type="hidden" value="i7s12i0" form="i7s12i04"> <input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png"> <input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled=""> <div id="i7s12i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i01" class="C1_text"> We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp-aiiA, sfgfp-aiiA-ndh. SDS-PAGE gel pictures are shown as follows.
"
