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Exosome Mediated Mammalian Cell-Cell Communication

Overview

miRNA Signal

Protein Signals

Novel DNA Sensor: Cas9 Split Venus Fusion

Our BioBricks

Attributions

Biobricks Favorite Parts • Name: CMV_Acyl-TyA-eGFP MammoBlock o ID: BBa_K1179071 o Type: Generator o Length: 2834 o Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence. • Name: Hef1a_Cas9-VP16 MammoBlock o ID: BBa_K1179002 o Type: Generator o Length: 5141 o Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream. • Name: Hef1a_eYFP-4x451 MammoBlock o ID: BBa_K1179072 o Type: Generator o Length: 2085 o Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression. Other parts we like: Acyl-TyA BBa_K1179000 Acyl-TyA-eGFP BBa_K1179001 Hef1a_Cas9-VP16 BBa_K1179002 Acyl-TyA-mKate-His BBa_K1179003 Acyl-TyA-rtTA BBa_K1179004 Acyl-TyA-rtTA-His BBa_K1179005 Acyl-TyA-Tev BBa_K1179006 Acyl-TyA-TEV-rtTA-His BBa_K1179007 Cas9 D10A & H840A mutation-linker BBa_K1179008 Cas9-Linker-VP16 BBa_K1179009 eBFP BBa_K1179010 eYFP BBa_K1179012 eYFP-PEST BBa_K1179013 VP16-GAL4 BBa_K1179014 U6_gRNA (Cr9) BBa_K1179015 L7Ae BBa_K1179016 Linker-Cas9 D10A & H840A mutation BBa_K1179017 mKate –PEST BBa_K1179018 mKate-His BBa_K1179019 rtTA-His BBa_K1179020 CAG_Acyl-TyA-mKate-His BBa_K1179021 CAG_Acyl-TyA-rtTA BBa_K1179022 CAG_Acyl-TyA-rtTA-His BBa_K1179023 CAG_eYFP-PEST BBa_K1179024 CAG_L7Ae BBa_K1179025 CAG_mKate-PEST BBa_K1179026 CAG-tagBFP BBa_K1179027 D10A and H840A mutant Cas9-VC155 BBa_K1179028 D10A and H840A mutant Cas9-VN155 BBa_K1179029 D10A and H840A mutant Cas9-VN173 BBa_K1179030 CMV_ L7Ae_myc-His6 BBa_K1179032 CMV_2xKMet-eGFP BBa_K1179033 L7Ae-2a-eYFP BBa_K1179037 TRE_Cas9-VP16 BBa_K1179040 U6_gRNA 1A Reverse BBa_K1179041 U6_gRNA 1A BBa_K1179042 U6_gRNA 2A BBa_K1179043 U6_gRNA 3A BBa_K1179044 U6_gRNA 4A BBa_K1179045 U6_gRNA 5A BBa_K1179046 U6_gRNA 6A BBa_K1179047 U6_gRNA 6A Reverse BBa_K1179048 VC155 BBa_K1179049 VC155 with Leucine zipper BBa_K1179050 VC155-Cas9(D10A and H840A mutant) BBa_K1179051 VN155 BBa_K1179052 VN155 with Leucine zipper BBa_K1179053 VN155-Cas9(D10A and H840A mutant) BBa_K1179054 VN173 BBa_K1179055 VN173 with Leucine zipper BBa_K1179056 VN173-Cas9(D10A and H840A mutant) BBa_K1179057 Cre entry vector BBa_K1179058 eYFP-4x451ts BBa_K1179060 eYFP-4x503ts BBa_K1179061 LacI BBa_K1179062 LacI-4x451ts BBa_K1179063 LacI-4x503ts BBa_K1179064 TagBFP BBa_K1179068 CAG BBa_K1179069 CMV_Acyl-TyA-eGFP BBa_K1179071 Hef1a_eYFP-4x451 BBa_K1179072 TRE-tight_eYFP-4x451 BBa_K1179073 Hef1a_eYFP-4x503 BBa_K1179074 TRE-t_eYFP-4x503 BBa_K1179075