Team:UT-Tokyo/test.html

From 2013.igem.org

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(34 intermediate revisions not shown)
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rel='stylesheet' type='text/css'>
rel='stylesheet' type='text/css'>
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<div id="global_wrapper"></div>
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<div id="global_wrapper">
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<div id="header"></div>
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<div id="header">
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<div class="logo">
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<div class="logo">
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<img src="https://static.igem.org/mediawiki/2013/9/93/Clock_ecoli_paper5.png"
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<img
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height="120px">
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src="https://static.igem.org/mediawiki/2013/9/93/Clock_ecoli_paper5.png"
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</div>
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height="120px">
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<div class="wrapper">
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</div>
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<div class="box">Thanks for your visiting our team wiki!</div>
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<div class="wrapper">
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</div>
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<div class="box">Thanks for your visiting our team wiki!</div>
 +
</div>
</html>
</html>
{{:Team:UT-Tokyo/Template/Header1|subpagename=TEAM}}
{{:Team:UT-Tokyo/Template/Header1|subpagename=TEAM}}
<html>
<html>
 +
<div id="contents">
 +
<h2>Automatic RNA silencing primer designer</h2>
-
<body>
 
<script type="text/javascript">
<script type="text/javascript">
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String.prototype.lengthByte = function() {
 
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var str = this;
 
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var r = 0;
 
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for ( var i = 0; i < str.length; i++) {
 
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var c = str.charCodeAt(i);
 
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if ((c >= 0x0 && c < 0x81) || (c == 0xf8f0)
 
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|| (c >= 0xff61 && c < 0xffa0)
 
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|| (c >= 0xf8f1 && c < 0xf8f4)) {
 
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r += 1;
 
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} else {
 
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r += 2;
 
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}
 
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}
 
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return r;
 
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}
 
-
 
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String.prototype.repeat = function(n) {
 
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return new Array(n + 1).join(this);
 
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}
 
-
 
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String.prototype.replaceAll = function(str1, str2) {
 
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var temp = this;
 
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while (temp.indexOf(str1) != -1) {
 
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temp = temp.replace(str1, str2);
 
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}
 
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;
 
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return temp;
 
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};
 
-
 
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function getWrappedText(str) {
 
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var len = Math.floor(str.lengthByte() / 2);
 
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return "＿" + ("人".repeat(len + 2)) + "＿<br />" + "＞　" + str
 
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+ "　＜<br />" + "￣Y" + ("^Y".repeat(len)) + "￣";
 
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}
 
-
 
function getReverseSeq(str) {
function getReverseSeq(str) {
//先頭24塩基を抽出してアンチセンスを返す
//先頭24塩基を抽出してアンチセンスを返す
Line 95: Line 62:
function getRP(str) {
function getRP(str) {
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var anti = getReverseSeq(str);
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var reverse = str.substring(7, 24);
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var reverse = str.substring(7, 24) + getReverseSeq("tactagag")
+
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+ getReverseSeq("acctctggcggtgataatggttgc");
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return reverse;
return reverse;
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}
 
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function getForwardOnly(str) {
 
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var anti = getReverseSeq(str);
 
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anti = "ggatctagag" + anti + "tttctgttgggccattgcattgcc";
 
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return anti;
 
}
}
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function onClick() {
function onClick() {
document.getElementById('sequence').innerHTML = "5'- "
document.getElementById('sequence').innerHTML = "5'- "
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+ document.gen.argtext.value + " -3' (CDS)";
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+ document.gen.argtext.value + " -3'";
var reverse = getReverse(document.gen.argtext.value);
var reverse = getReverse(document.gen.argtext.value);
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while (reverse.length < 34) {
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var forwardonly = "<p style=\"display:inline;color: #3CB371;\">ggatctagag</p>"
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reverse = "-" + reverse;
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+ getReverseSeq(document.gen.argtext.value)
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}
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+ "<p style=\"display:inline;color: #1E90FF;\">tttctgttgggccattgcattgcc</p>";
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document.getElementById('reverse').innerHTML = "3'- " + reverse
+
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+ " -5' (CDS)";
+
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var forwardonly = getForwardOnly(document.gen.argtext.value);
+
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var interaction = "--------------||||||||||||||||||||||||";
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while (forwardonly.length - reverse.length < 24) {
+
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forwardonly = "-" + forwardonly;
+
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interaction = "-" + interaction;
+
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}
+
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document.getElementById('interaction').innerHTML = interaction;
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document.getElementById('generated').innerHTML = "5'- "
document.getElementById('generated').innerHTML = "5'- "
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+ forwardonly + " -3' (Forward Primer for BioBrick)";
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+ forwardonly + " -3'";
 +
}
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var fp = "---------------------------------------"
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function onClick2() {
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+ getFP(document.gen.argtext.value);
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document.getElementById('sequence2').innerHTML = "5'- "
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var int2 = "-------------------------------------------||||||||||";
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+ document.gen2.argtext2.value + " -3'";
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var rp = getRP(document.gen.argtext.value);
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document.getElementById('fp').innerHTML = "5'- " + fp
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var fp = getFP(document.gen2.argtext2.value);
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+ " -3' (Forward Primer for Inverse PCR)";
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var rp = getRP(document.gen2.argtext2.value)
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document.getElementById('int2').innerHTML = int2;
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+ getReverseSeq("tactagag")
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document.getElementById('rp').innerHTML = "5'- " + rp
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+ getReverseSeq("acctctggcggtgataatggttgc");
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+ " -3' (Reverse Primer for Inverse PCR)";
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document.getElementById('fp').innerHTML = "5'- " + fp + " -3'";
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document.getElementById('rp_rev').innerHTML = "3'- "
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document.getElementById('rp').innerHTML = "5'- " + rp + " -3'";
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+ getReverse(rp) + " -5' (Reverse Primer for Inverse PCR)";
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}
}
 +
function submitStop(e) {
 +
if (!e)
 +
var e = window.event;
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function tweet() {
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if (e.keyCode == 13)
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window.open("https://twitter.com/intent/tweet?text="
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return false;
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+ encodeURIComponent(getReverseSeq(
+
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document.gen.argtext.value).replaceAll("<br />",
+
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"\n")
+
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+ "\n")
+
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+ "&url=http://kenkoooo.com/wikifreeze.html");
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}
}
</script>
</script>
<form name="gen">
<form name="gen">
<input type="text" name="argtext"
<input type="text" name="argtext"
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value="ATGCATGCATGCATGCATGCATGCATGCATGC" /> <input type="button"
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value="ATGCATGCATGCATGCATGCATGCATGCATGC"
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value="generate" onclick="onClick()" />
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onKeyPress="onClick();return submitStop(event);" /> <input
 +
type="button" value="generate" onclick="onClick()" />
</form>
</form>
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<div style="font-family: monospace;">
+
 
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<p id="sequence">Wiki Freeze</p>
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<div>
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<p id="reverse" style="margin: 0px;">Wiki Freeze</p>
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<h3>CDS</h3>
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<p id="interaction" style="margin: 0px;">Wiki Freeze</p>
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<p id="sequence"></p>
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<p id="generated" style="margin: 0px;">Wiki Freeze</p>
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<h3>Forward Primer for BioBrick</h3>
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<p></p>
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<p id="generated"></p>
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<p id="fp" style="margin: 0px;">Wiki Freeze</p>
+
-
<p id="int2" style="margin: 0px;">Wiki Freeze</p>
+
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<p id="rp_rev" style="margin: 0px;">Wiki Freeze</p>
+
-
<p id="rp">Wiki Freeze</p>
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</div>
</div>
 +
<form name="gen2">
 +
<input type="text" name="argtext2"
 +
value="ATGCATGCATGCATGCATGCATGCATGCATGC"
 +
onKeyPress="onClick2();return submitStop(event);" /> <input
 +
type="button" value="generate" onclick="onClick2()" />
 +
</form>
 +
<div>
 +
<h3>CDS</h3>
 +
<p id="sequence2"></p>
 +
<h3>Forward Primer for Inverse PCR</h3>
 +
<p id="fp"></p>
 +
<h3>Reverse Primer for Inverse PCR</h3>
 +
<p id="rp"></p>
 +
</div>
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<div id="footer"></div>
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</div>
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</body>
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</div>
 +
<div id="footer"></div>
 +
 +
</div>
 +
<p>
</html>
</html>

Latest revision as of 03:34, 28 September 2013

           TEAM

Automatic RNA silencing primer designer

CDS

Forward Primer for BioBrick

CDS

Forward Primer for Inverse PCR

Reverse Primer for Inverse PCR

Retrieved from "http://2013.igem.org/Team:UT-Tokyo/test.html"