Team:UT-Tokyo/test.html

From 2013.igem.org

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<html>
<html>
<div id="contents">
<div id="contents">
-
aaaaa
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<h2>Automatic RNA silencing primer designer</h2>
 +
 
 +
 
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<script type="text/javascript">
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function getReverseSeq(str) {
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//先頭24塩基を抽出してアンチセンスを返す
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var rev = "";
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var min = 24;
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if (str.length < 24) {
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min = str.length;
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}
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for ( var i = 0; i < min; i++) {
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var c = str.charAt(i);
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if (c.indexOf("A") !== -1) {
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c = "T";
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} else if (c.indexOf("T") !== -1) {
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c = "A";
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} else if (c.indexOf("C") !== -1) {
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c = "G";
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} else if (c.indexOf("G") !== -1) {
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c = "C";
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} else if (c.indexOf("a") !== -1) {
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c = "t";
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} else if (c.indexOf("t") !== -1) {
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c = "a";
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} else if (c.indexOf("c") !== -1) {
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c = "g";
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} else if (c.indexOf("g") !== -1) {
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c = "c";
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}
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rev = c + rev;
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}
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return rev;
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}
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function getFP(str) {
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var anti = getReverseSeq(str);
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var forward = anti.substring(7, 24) + "tttctgttgggccattgcattgcc";
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return forward;
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}
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function getRP(str) {
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var reverse = str.substring(7, 24);
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return reverse;
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}
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function getReverse(str) {
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var rev = "";
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for ( var i = 0; i < str.length; i++) {
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var c = str.charAt(i);
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rev = c + rev;
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}
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return rev;
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}
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function onClick() {
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document.getElementById('sequence').innerHTML = "5'- "
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+ document.gen.argtext.value + " -3'";
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var reverse = getReverse(document.gen.argtext.value);
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var forwardonly = "<p style=\"display:inline;color: #3CB371;\">ggatctagag</p>"
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+ getReverseSeq(document.gen.argtext.value)
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+ "<p style=\"display:inline;color: #1E90FF;\">tttctgttgggccattgcattgcc</p>";
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document.getElementById('generated').innerHTML = "5'- "
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+ forwardonly + " -3'";
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}
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function onClick2() {
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document.getElementById('sequence2').innerHTML = "5'- "
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+ document.gen2.argtext2.value + " -3'";
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var fp = getFP(document.gen2.argtext2.value);
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var rp = getRP(document.gen2.argtext2.value)
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+ getReverseSeq("tactagag")
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+ getReverseSeq("acctctggcggtgataatggttgc");
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document.getElementById('fp').innerHTML = "5'- " + fp + " -3'";
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document.getElementById('rp').innerHTML = "5'- " + rp + " -3'";
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}
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function submitStop(e) {
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if (!e)
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var e = window.event;
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if (e.keyCode == 13)
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return false;
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}
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</script>
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<form name="gen">
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<input type="text" name="argtext"
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value="ATGCATGCATGCATGCATGCATGCATGCATGC"
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onKeyPress="onClick();return submitStop(event);" /> <input
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type="button" value="generate" onclick="onClick()" />
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</form>
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<div>
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<h3>CDS</h3>
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<p id="sequence"></p>
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<h3>Forward Primer for BioBrick</h3>
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<p id="generated"></p>
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</div>
 +
 
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<form name="gen2">
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<input type="text" name="argtext2"
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value="ATGCATGCATGCATGCATGCATGCATGCATGC"
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onKeyPress="onClick2();return submitStop(event);" /> <input
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type="button" value="generate" onclick="onClick2()" />
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</form>
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<div>
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<h3>CDS</h3>
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<p id="sequence2"></p>
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<h3>Forward Primer for Inverse PCR</h3>
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<p id="fp"></p>
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<h3>Reverse Primer for Inverse PCR</h3>
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<p id="rp"></p>
 +
</div>
</div>
</div>
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</div>
</div>
<p>
<p>
-
 
</html>
</html>

Latest revision as of 03:34, 28 September 2013

           TEAM

Automatic RNA silencing primer designer

CDS

Forward Primer for BioBrick

CDS

Forward Primer for Inverse PCR

Reverse Primer for Inverse PCR

Retrieved from "http://2013.igem.org/Team:UT-Tokyo/test.html"