Team:UT-Tokyo/test.html

From 2013.igem.org

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{{:Team:UT-Tokyo/Template/Header1|subpagename=TEAM}}
{{:Team:UT-Tokyo/Template/Header1|subpagename=TEAM}}
<html>
<html>
-
<div id="contents"><script type="text/javascript">
+
<div id="contents">
 +
<h2>Automatic RNA silencing primer designer</h2>
 +
 
 +
 
 +
<script type="text/javascript">
function getReverseSeq(str) {
function getReverseSeq(str) {
//先頭24塩基を抽出してアンチセンスを返す
//先頭24塩基を抽出してアンチセンスを返す
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function getRP(str) {
function getRP(str) {
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var anti = getReverseSeq(str);
+
var reverse = str.substring(7, 24);
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var reverse = str.substring(7, 24) + getReverseSeq("tactagag")
+
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+ getReverseSeq("acctctggcggtgataatggttgc");
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return reverse;
return reverse;
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}
 
-
 
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function getForwardOnly(str) {
 
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var anti = getReverseSeq(str);
 
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anti = "ggatctagag" + anti + "tttctgttgggccattgcattgcc";
 
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return anti;
 
}
}
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function onClick() {
function onClick() {
document.getElementById('sequence').innerHTML = "5'- "
document.getElementById('sequence').innerHTML = "5'- "
-
+ document.gen.argtext.value + " -3' (CDS)";
+
+ document.gen.argtext.value + " -3'";
var reverse = getReverse(document.gen.argtext.value);
var reverse = getReverse(document.gen.argtext.value);
-
while (reverse.length < 34) {
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var forwardonly = "<p style=\"display:inline;color: #3CB371;\">ggatctagag</p>"
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reverse = "-" + reverse;
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+ getReverseSeq(document.gen.argtext.value)
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}
+
+ "<p style=\"display:inline;color: #1E90FF;\">tttctgttgggccattgcattgcc</p>";
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document.getElementById('reverse').innerHTML = "3'- " + reverse
+
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+ " -5' (CDS)";
+
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var forwardonly = getForwardOnly(document.gen.argtext.value);
+
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var interaction = "--------------||||||||||||||||||||||||";
+
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while (forwardonly.length - reverse.length < 24) {
+
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forwardonly = "-" + forwardonly;
+
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interaction = "-" + interaction;
+
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}
+
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document.getElementById('interaction').innerHTML = interaction;
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document.getElementById('generated').innerHTML = "5'- "
document.getElementById('generated').innerHTML = "5'- "
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+ forwardonly + " -3' (Forward Primer for BioBrick)";
+
+ forwardonly + " -3'";
 +
}
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var fp = "---------------------------------------"
+
function onClick2() {
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+ getFP(document.gen.argtext.value);
+
document.getElementById('sequence2').innerHTML = "5'- "
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var int2 = "-------------------------------------------||||||||||";
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+ document.gen2.argtext2.value + " -3'";
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var rp = getRP(document.gen.argtext.value);
+
 
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document.getElementById('fp').innerHTML = "5'- " + fp
+
var fp = getFP(document.gen2.argtext2.value);
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+ " -3' (Forward Primer for Inverse PCR)";
+
var rp = getRP(document.gen2.argtext2.value)
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document.getElementById('int2').innerHTML = int2;
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+ getReverseSeq("tactagag")
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document.getElementById('rp').innerHTML = "5'- " + rp
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+ getReverseSeq("acctctggcggtgataatggttgc");
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+ " -3' (Reverse Primer for Inverse PCR)";
+
document.getElementById('fp').innerHTML = "5'- " + fp + " -3'";
-
document.getElementById('rp_rev').innerHTML = "3'- "
+
document.getElementById('rp').innerHTML = "5'- " + rp + " -3'";
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+ getReverse(rp) + " -5' (Reverse Primer for Inverse PCR)";
+
}
 +
function submitStop(e) {
 +
if (!e)
 +
var e = window.event;
 +
 
 +
if (e.keyCode == 13)
 +
return false;
}
}
</script>
</script>
<form name="gen">
<form name="gen">
<input type="text" name="argtext"
<input type="text" name="argtext"
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value="ATGCATGCATGCATGCATGCATGCATGCATGC" /> <input type="button"
+
value="ATGCATGCATGCATGCATGCATGCATGCATGC"
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value="generate" onclick="onClick()" />
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onKeyPress="onClick();return submitStop(event);" /> <input
 +
type="button" value="generate" onclick="onClick()" />
</form>
</form>
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<div style="font-family: monospace;">
+
 
 +
<div>
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<h3>CDS</h3>
<p id="sequence"></p>
<p id="sequence"></p>
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<p id="reverse" style="margin: 0px;"></p>
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<h3>Forward Primer for BioBrick</h3>
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<p id="interaction" style="margin: 0px;"></p>
+
<p id="generated"></p>
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<p id="generated" style="margin: 0px;"></p>
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</div>
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<p></p>
+
 
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<p id="fp" style="margin: 0px;"></p>
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<form name="gen2">
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<p id="int2" style="margin: 0px;"></p>
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<input type="text" name="argtext2"
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<p id="rp_rev" style="margin: 0px;"></p>
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value="ATGCATGCATGCATGCATGCATGCATGCATGC"
 +
onKeyPress="onClick2();return submitStop(event);" /> <input
 +
type="button" value="generate" onclick="onClick2()" />
 +
</form>
 +
 
 +
<div>
 +
<h3>CDS</h3>
 +
<p id="sequence2"></p>
 +
<h3>Forward Primer for Inverse PCR</h3>
 +
<p id="fp"></p>
 +
<h3>Reverse Primer for Inverse PCR</h3>
<p id="rp"></p>
<p id="rp"></p>
</div>
</div>
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</div>
</div>
<p>
<p>
-
 
</html>
</html>

Latest revision as of 03:34, 28 September 2013

           TEAM

Automatic RNA silencing primer designer

CDS

Forward Primer for BioBrick

CDS

Forward Primer for Inverse PCR

Reverse Primer for Inverse PCR

Retrieved from "http://2013.igem.org/Team:UT-Tokyo/test.html"