Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
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===Electrophoresis=== | ===Electrophoresis=== | ||
- | |||
<div class="experiment"> | <div class="experiment"> | ||
- | <span class="author">Nakamoto</span> | + | <span class="author">Tatsui and Nakamoto</span> |
{| class="wikitable" | {| class="wikitable" | ||
- | ! | + | !Lane||Sample |
|- | |- | ||
- | | | + | | ||100bp ladder |
|- | |- | ||
- | |8/9 | + | |4||8/9 BBa_J44000 -1 |
|- | |- | ||
- | |8/9 | + | |5||8/9 BBa_J44000 -2 |
|- | |- | ||
- | | | + | |6||NC |
+ | |} | ||
+ | [[File:Igku Aug10 Electrophoresis BBa 1.jpg]]<br> | ||
+ | {| class="wikitable" | ||
+ | !Lane||Sample | ||
|- | |- | ||
- | |8/9 | + | | ||1kbp ladder |
+ | |- | ||
+ | |1||8/9 BBa_J23106 | ||
+ | |- | ||
+ | |2||8/9 BBa_J23100 -1 | ||
+ | |- | ||
+ | |3||8/9 BBa_J23100 -2 | ||
+ | |- | ||
+ | |4||NC | ||
|} | |} | ||
+ | [[File:Igku Aug10electrophoresis1.jpg]]<br> | ||
</div> | </div> | ||
- | === | + | ===Liquid Culture=== |
<div class="experiment"> | <div class="experiment"> | ||
<span class="author">Nakamoto</span> | <span class="author">Nakamoto</span> | ||
{| class="wikitable" | {| class="wikitable" | ||
- | !Sample | + | !Sample||medium |
|- | |- | ||
- | |BBa- | + | |8/9 BBa-J23106||LB(+Amp) |
|- | |- | ||
- | |BBa- | + | |8/9 BBa-J23100-1||LB(+Amp) |
|- | |- | ||
- | |BBa- | + | |8/9 BBa-J23100-2||LB(+Amp) |
|- | |- | ||
- | |BBa- | + | |8/9 BBa-J44000-1||LB(+Amp) |
|- | |- | ||
- | |BBa- | + | |8/9 BBa-J44000-2||LB(+Amp) |
|} | |} | ||
</div> | </div> | ||
Line 90: | Line 102: | ||
|0.4M NaOHaq||500µL | |0.4M NaOHaq||500µL | ||
|- | |- | ||
- | |Agarose Pouder|| | + | |Agarose Pouder||2g |
|- | |- | ||
|Ampicillin||40µL | |Ampicillin||40µL | ||
Line 113: | Line 125: | ||
|} | |} | ||
</div> | </div> | ||
- | |||
- |
Latest revision as of 03:40, 28 September 2013
Contents |
Aug 10
Colony PCR
Sample |
---|
8/9 BBa_J44000 -(1) |
8/9 BBa_J44000 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 30 sec | 30 cycles |
Sample |
---|
8/9 BBa_J23106 |
8/9 BBa_J23100 -(1) |
8/9 BBa_J23100 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 1 min | 30 cycles |
Electrophoresis
Lane | Sample |
---|---|
100bp ladder | |
4 | 8/9 BBa_J44000 -1 |
5 | 8/9 BBa_J44000 -2 |
6 | NC |
Lane | Sample |
---|---|
1kbp ladder | |
1 | 8/9 BBa_J23106 |
2 | 8/9 BBa_J23100 -1 |
3 | 8/9 BBa_J23100 -2 |
4 | NC |
Liquid Culture
Sample | medium |
---|---|
8/9 BBa-J23106 | LB(+Amp) |
8/9 BBa-J23100-1 | LB(+Amp) |
8/9 BBa-J23100-2 | LB(+Amp) |
8/9 BBa-J44000-1 | LB(+Amp) |
8/9 BBa-J44000-2 | LB(+Amp) |
LB Medium Plate(+Ampicillin)
volume | 200ml |
---|---|
Bacto T2ypton | 2g |
Bacto yeast extract | 1g |
NaCl | 1g |
0.4M NaOHaq | 500µL |
Agarose Pouder | 2g |
Ampicillin | 40µL |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
tetR | 3µL | 19µL | 22µL | Kan |
RBS | 3µL | 19µL | 22µL | Amp |
lacZα | 3µL | 19µL | 22µL | CP |
lacI | 3µL | 19µL | 22µL | CP |
Plac | 3µL | 19µL | 22µL | CP |