Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
(Difference between revisions)
(âElectrophoresis) |
(âLB Medium Plate(+Ampicillin)) |
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Line 76: | Line 76: | ||
!Sample||medium | !Sample||medium | ||
|- | |- | ||
- | |8/9 BBa-J23106||LB(+ | + | |8/9 BBa-J23106||LB(+Amp) |
|- | |- | ||
- | |8/9 BBa-J23100-1||LB(+ | + | |8/9 BBa-J23100-1||LB(+Amp) |
|- | |- | ||
- | |8/9 BBa-J23100-2||LB(+ | + | |8/9 BBa-J23100-2||LB(+Amp) |
|- | |- | ||
- | |8/9 BBa-J44000-1||LB(+ | + | |8/9 BBa-J44000-1||LB(+Amp) |
|- | |- | ||
- | |8/9 BBa-J44000-2||LB(+ | + | |8/9 BBa-J44000-2||LB(+Amp) |
|} | |} | ||
</div> | </div> | ||
Line 102: | Line 102: | ||
|0.4M NaOHaq||500µL | |0.4M NaOHaq||500µL | ||
|- | |- | ||
- | |Agarose Pouder|| | + | |Agarose Pouder||2g |
|- | |- | ||
|Ampicillin||40µL | |Ampicillin||40µL |
Latest revision as of 03:40, 28 September 2013
Contents |
Aug 10
Colony PCR
Sample |
---|
8/9 BBa_J44000 -(1) |
8/9 BBa_J44000 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 30 sec | 30 cycles |
Sample |
---|
8/9 BBa_J23106 |
8/9 BBa_J23100 -(1) |
8/9 BBa_J23100 -(2) |
NC |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94 °C | 94 °C | 55 °C | 68 °C | -- |
5 min | 30 sec | 30 sec | 1 min | 30 cycles |
Electrophoresis
Lane | Sample |
---|---|
100bp ladder | |
4 | 8/9 BBa_J44000 -1 |
5 | 8/9 BBa_J44000 -2 |
6 | NC |
Lane | Sample |
---|---|
1kbp ladder | |
1 | 8/9 BBa_J23106 |
2 | 8/9 BBa_J23100 -1 |
3 | 8/9 BBa_J23100 -2 |
4 | NC |
Liquid Culture
Sample | medium |
---|---|
8/9 BBa-J23106 | LB(+Amp) |
8/9 BBa-J23100-1 | LB(+Amp) |
8/9 BBa-J23100-2 | LB(+Amp) |
8/9 BBa-J44000-1 | LB(+Amp) |
8/9 BBa-J44000-2 | LB(+Amp) |
LB Medium Plate(+Ampicillin)
volume | 200ml |
---|---|
Bacto T2ypton | 2g |
Bacto yeast extract | 1g |
NaCl | 1g |
0.4M NaOHaq | 500µL |
Agarose Pouder | 2g |
Ampicillin | 40µL |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
tetR | 3µL | 19µL | 22µL | Kan |
RBS | 3µL | 19µL | 22µL | Amp |
lacZα | 3µL | 19µL | 22µL | CP |
lacI | 3µL | 19µL | 22µL | CP |
Plac | 3µL | 19µL | 22µL | CP |