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Team:UIUC Illinois/Project/Parts
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<ul> | <ul> | ||
<li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Overview">Overview</a></li> | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Overview">Overview</a></li> | ||
| - | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Background_Info">Background | + | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Background_Info">Background</a></li> |
<li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Design">Design</a></li> | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Design">Design</a></li> | ||
<li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Parts"><u>Parts</u></a></li> | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Project/Parts"><u>Parts</u></a></li> | ||
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<h3>Human Practices</h3> | <h3>Human Practices</h3> | ||
<ul> | <ul> | ||
| - | |||
<li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Human_Practices/Outreach">Outreach</a></li> | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Human_Practices/Outreach">Outreach</a></li> | ||
<li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Human_Practices/Ethics">Ethics</a></li> | <li style="margin-bottom:20px;"><a href="https://2013.igem.org/Team:UIUC_Illinois/Human_Practices/Ethics">Ethics</a></li> | ||
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<div id="container" class="left"> | <div id="container" class="left"> | ||
<div id="major_image"> | <div id="major_image"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/e/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e1/UIUC_Plasmid_Maps%21%21%21.PNG" width="720" height="165" alt="plasmid maps" /> |
</div> | </div> | ||
<p class="center"> | <p class="center"> | ||
<b>Cardiobiotics Parts </b><br></p> | <b>Cardiobiotics Parts </b><br></p> | ||
| - | <p class="left"> <b> | + | <p class="left"> <b>Carnitine dehydrogenase (cdhCAB) BBa_K1205000 </b><br> |
| - | • Derived from <i> | + | • Derived from <i>Pseudomonas aeruginosa PAO1</i> <br> |
• This gene codes for L-Carnitine Dehydrogenase, a protein that breaks down L-carnitine into 3-Dehydrocarnitine. This enzyme directs the degradation down a pathway that prevents the formation of trimethylamine. As previously mentioned trimethylamine has been known to cause cardiovascular disease. This new pathway ultimately leads to glycine which is a nature substance in the body. This enzyme is the centerpiece of our experiment. <br> | • This gene codes for L-Carnitine Dehydrogenase, a protein that breaks down L-carnitine into 3-Dehydrocarnitine. This enzyme directs the degradation down a pathway that prevents the formation of trimethylamine. As previously mentioned trimethylamine has been known to cause cardiovascular disease. This new pathway ultimately leads to glycine which is a nature substance in the body. This enzyme is the centerpiece of our experiment. <br> | ||
Characterization: <br> | Characterization: <br> | ||
• This part was characterized with an enzymatic assay. | • This part was characterized with an enzymatic assay. | ||
| + | </p> | ||
| + | <img style="-webkit-user-select: none" src="https://static.igem.org/mediawiki/2013/f/fd/UIUC_cdh_gelelec.jpg"> | ||
| + | <p> | ||
<br> | <br> | ||
<br> | <br> | ||
| - | <b> | + | <b>cbcVW BBa_K1205002</b> <br> |
• From <i>pseudomonas aeruginosa</i> strain PAO1 <br> | • From <i>pseudomonas aeruginosa</i> strain PAO1 <br> | ||
• Codes for a membrane bound protein that allows for the transport of L-carnitine into the cell. <br> | • Codes for a membrane bound protein that allows for the transport of L-carnitine into the cell. <br> | ||
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Characterization: <br> | Characterization: <br> | ||
Proof of functionality: LCMS <br> | Proof of functionality: LCMS <br> | ||
| + | </p> | ||
| + | <img style="-webkit-user-select: none" src="https://static.igem.org/mediawiki/2013/3/3d/UIUC_cbcvw_gelelectro.jpg"> | ||
| + | <p> | ||
<br> | <br> | ||
| - | <b> | + | <b>caiX BBa_K1205001</b> <br> |
• From <i>pseudomonas aeruginosa</i> strain PAO1<br> | • From <i>pseudomonas aeruginosa</i> strain PAO1<br> | ||
• Codes for a transport protein that allows for L-carnitine to be directed to the membrane bound channel to then be transported into the cell. <br> | • Codes for a transport protein that allows for L-carnitine to be directed to the membrane bound channel to then be transported into the cell. <br> | ||
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Characterization: <br> | Characterization: <br> | ||
Proof of functionality: LCMS <br> | Proof of functionality: LCMS <br> | ||
| + | </p> | ||
| + | <img style="-webkit-user-select: none" src="https://static.igem.org/mediawiki/2013/2/2b/UIUC_caix_gelelect.jpg"> | ||
| + | <p> | ||
| + | |||
<br> | <br> | ||
| - | <b> | + | <b>cbcX</b> <br> |
• From <i>pseudomonas aeruginosa</i> strain PAO1 <br> | • From <i>pseudomonas aeruginosa</i> strain PAO1 <br> | ||
• Codes for a transport protien that takes choline to a transport channel to increase rate of choline uptake. <br> | • Codes for a transport protien that takes choline to a transport channel to increase rate of choline uptake. <br> | ||
Latest revision as of 03:47, 28 September 2013
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Cardiobiotics Parts
Carnitine dehydrogenase (cdhCAB) BBa_K1205000
• Derived from Pseudomonas aeruginosa PAO1
• This gene codes for L-Carnitine Dehydrogenase, a protein that breaks down L-carnitine into 3-Dehydrocarnitine. This enzyme directs the degradation down a pathway that prevents the formation of trimethylamine. As previously mentioned trimethylamine has been known to cause cardiovascular disease. This new pathway ultimately leads to glycine which is a nature substance in the body. This enzyme is the centerpiece of our experiment.
Characterization:
• This part was characterized with an enzymatic assay.
cbcVW BBa_K1205002
• From pseudomonas aeruginosa strain PAO1
• Codes for a membrane bound protein that allows for the transport of L-carnitine into the cell.
• This is crucial to our project because if we want to implement this as a system we need the cell to have the ability to uptake an excess of L-carnitine.
• Proof of functionality: LCMS Assay
Characterization:
Proof of functionality: LCMS
caiX BBa_K1205001
• From pseudomonas aeruginosa strain PAO1
• Codes for a transport protein that allows for L-carnitine to be directed to the membrane bound channel to then be transported into the cell.
• This is crucial to our project because if we want to implement this as a system we need the cell to have the ability to uptake an excess of L-carnitine.
• Proof of functionality: LCMS Assay
Characterization:
Proof of functionality: LCMS
cbcX
• From pseudomonas aeruginosa strain PAO1
• Codes for a transport protien that takes choline to a transport channel to increase rate of choline uptake.
• This is useful to our project because choline is also a compound that can be degraded to TMA. If we also modified its degradation our probiotic would be even more effective in decreasing risk for CVD. This gene would help increase uptake to maximize our probiotic's effectiveness.
• Unfortunately, due to the constraints of time and money, this developed biobrick could not be characterized
