Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.26T7Titer

From 2013.igem.org

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: The titer of T7 phage showed plaques down to a -5 concentration.  This concentration will allow us to perform and EM and examine how pure the phage solution is.
: The titer of T7 phage showed plaques down to a -5 concentration.  This concentration will allow us to perform and EM and examine how pure the phage solution is.
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[[File:Phage_Titer_6.26.13| thumb|none|alt=A phage plaques were found but with contamination.|T7 Titer]]
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[[File:Phage_Titer_6.26.13.jpg| thumb|none|alt=A phage plaques were found but with contamination.|T7 Titer]]

Revision as of 20:53, 3 July 2013


Phage Purification May - June Notebook: Experiments



Overview
March-April
May-June
July-August
September-October

6.26 Titer


I) Purpose

Test the concentration of purified T7 phage.

II) Expected Outcome

Plaques from the bands in the CsCl gradient where phage were purified. Expecting plaques down to at least -5 concentration

III) Reagants Used

BL21 freezer stock
LB
1.5x top agar
T7 phage from CsCl gradient


IV) Actual Procedure

Infect 500 µL of LB with 1 loop-full of BL21 freezer stock and put on shaker at 37◦ C for 5 hours.
Plate BL21 on an LB plate with 4.5 mL top agar.
Dilute T7 using a dilution series.
Spot 2 µL of phage from dilution series onto plate using the concentrations 0, -2, -3, -4, -5, and -6.
Place in 37◦ C incubator overnight.

V) Results

The titer of T7 phage showed plaques down to a -5 concentration. This concentration will allow us to perform and EM and examine how pure the phage solution is.
A phage plaques were found but with contamination.
T7 Titer


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