Team:Manchester/fattytest

From 2013.igem.org

(Difference between revisions)
 
(21 intermediate revisions not shown)
Line 84: Line 84:
left:0;
left:0;
padding:10px;
padding:10px;
-
background-color:#ccc;
+
background-color:white;
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
Line 92: Line 92:
}
}
-
.block1 a, .block2 a, .block3 a, .block4 a
+
.container1
 +
{
 +
margin:10px;
 +
width:900px;
 +
padding:10px;
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text1
 +
{
 +
margin:10px;
 +
width:900px;
 +
font-style:Trebuchet MS;
 +
font-size:14px;
 +
color:#4c0082;
 +
background-color:#F2F2F2;
 +
padding:10px;
 +
 
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text1 p
 +
{
 +
font-style:Trebuchet MS;
 +
font-size:14px;
 +
color:#4c0082;
 +
background-color:#F2F2F2;
 +
}
 +
 
 +
.text1 img
 +
{
 +
float:right;
 +
margin:10px;
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text2
 +
{
 +
margin:10px;
 +
width:900px;
 +
font-style:Trebuchet MS;
 +
font-size:14px;
 +
color:#4c0082;
 +
background-color:#F2F2F2;
 +
padding:10px;
 +
 
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text2 p
 +
{
 +
font-style:Trebuchet MS;
 +
font-size:14px;
 +
color:#4c0082;
 +
background-color:#F2F2F2;
 +
 
 +
}
 +
 
 +
.text2 img
 +
{
 +
float:left;
 +
margin:10px;
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text3
 +
{
 +
margin:10px;
 +
width:900px;
 +
font-style:Trebuchet MS;
 +
font-size:14px;
 +
color:#4c0082;
 +
background-color:#F2F2F2;
 +
padding:10px;
 +
 
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.text3 img
 +
{
 +
 
 +
-webkit-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
-moz-box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
box-shadow: 0px 0px 5px 0px rgba(0,0,0,0.75);
 +
}
 +
 
 +
.block1 a, .block2 a, .block3 a, .block4 a, .block5 a, .block6 a, .block7 a, .block8 a, .block9 a, .block10 a, .block11 a, .block12 a, .block13 a
{
{
width:120px;
width:120px;
Line 99: Line 197:
text-align:center;
text-align:center;
background:#606060;
background:#606060;
-
padding:15px;
+
padding:5px;
font-style:Trebuchet MS;
font-style:Trebuchet MS;
font-weight:bold;
font-weight:bold;
-
font-size:14px;
+
font-size:12px;
color:white;
color:white;
}
}
-
.block1 a
+
.block1 a:hover, .block2 a:hover, .block3 a:hover, .block4 a:hover, .block5 a:hover, .block6 a:hover, .block7 a:hover, .block8 a:hover, .block9 a:hover, .block10 a:hover, .block11 a:hover, .block12 a:hover, .block13 a:hover
-
{
+
-
background:#660099;
+
-
}
+
-
 
+
-
.block1 a:hover, .block2 a:hover, .block3 a:hover, .block4 a:hover
+
{
{
background:#C0C0C0;
background:#C0C0C0;
Line 117: Line 210:
}
}
-
.block1 a
+
.leftbar
{
{
 +
width:120px;
 +
height:600px;
position:fixed;
position:fixed;
-
top:250px;
+
top:120px;
left:5px;
left:5px;
 +
}
 +
 +
.block1 a
 +
{
 +
background:#660099;
 +
float:left;
 +
display:block;
 +
padding:5px;
}
}
Line 127: Line 230:
{
{
background:#bc80ea;
background:#bc80ea;
-
position:fixed;
+
display:block;
-
top:300px;
+
float:left;
-
left:5px;
+
margin-top:1px;
 +
padding:5px;
}
}
.block3 a
.block3 a
{
{
-
position:fixed;
+
 
-
top:370px;
+
display:block;
-
left:5px;
+
float:left;
 +
margin-top:1px;
 +
padding:5px;
}
}
.block4 a
.block4 a
{
{
 +
 +
display:block;
 +
float:left;
 +
margin-top:1px;
 +
padding:5px;
 +
}
 +
 +
.block5 a
 +
{
 +
 +
display:block;
 +
float:left;
 +
margin-top:1px;
 +
padding:5px;
 +
}
 +
 +
.block6 a
 +
{
 +
display:block;
 +
float:left;
 +
margin-top:1px;
 +
padding:5px;
 +
}
 +
 +
.rightbar
 +
{
 +
width:120px;
position:fixed;
position:fixed;
-
top:439px;
+
top:120px;
-
left:5px;
+
right:5px;
 +
}
 +
 
 +
.question1 a, .question2 a, .question3 a, .question4 a, .question5 a
 +
{
 +
width:120px;
 +
text-decoration:none;
 +
color:white;
 +
text-align:center;
 +
background:#606060;
 +
padding:5px;
 +
font-style:Trebuchet MS;
 +
font-weight:bold;
 +
font-size:12px;
 +
color:white;
 +
}
 +
 
 +
.question1 a:hover, .question2 a:hover, .question3 a:hover, .question4 a:hover, .question5 a:hover
 +
{
 +
background:#C0C0C0;
 +
color:#606060;
 +
}
 +
 
 +
.question1 a
 +
{
 +
margin-bottom:1px;
 +
float:left;
 +
display:block;
 +
padding:5px;
 +
}
 +
 
 +
.question2 a
 +
{
 +
margin-bottom:1px;
 +
float:left;
 +
display:block;
 +
padding:5px;
 +
}
 +
 
 +
.question3 a
 +
{
 +
margin-bottom:1px;
 +
float:left;
 +
display:block;
 +
padding:5px;
 +
}
 +
 
 +
.question4 a
 +
{
 +
margin-top:1px;
 +
float:left;
 +
display:block;
 +
padding:5px;
 +
}
 +
 
 +
.question5 a
 +
{
 +
 
 +
margin-top:1px;
 +
float:left;
 +
display:block;
 +
padding:5px;
 +
}
 +
 
 +
#Q1,#Q2,#Q3,#Q4,#Q5 
 +
{
 +
text-decoration:none;
 +
color:#660099;
 +
font-family:Trebuchet MS;
 +
font-size:14px;
}
}
Line 155: Line 357:
<div class="banner">
<div class="banner">
  <div class="igem">  
  <div class="igem">  
-
  <a>
+
  <a href="https://2013.igem.org/Main_Page">
           <img src="https://static.igem.org/mediawiki/2013/3/3f/Igem.png">
           <img src="https://static.igem.org/mediawiki/2013/3/3f/Igem.png">
           </a>
           </a>
Line 177: Line 379:
<div class="global">
<div class="global">
<div class="wrapper" >
<div class="wrapper" >
-
       
+
          <div class="text1">
 +
            <p><b><a id="Q1">Introduction</a></b><br>
 +
Since the model of the fatty acid biosynthesis pathways highlighted several key enzymes that could be altered to produce palm oil, including Delta9, Delta12 and FabA, which agrees with previous publications on these enzymes. Delta9 and Delta12 are involved in linear pathways, meaning that there are obvious reactants and products, so the overexpression of them can be measured directly via LC-MS and other techniques. However, FabA is a β-Hydroxydecanoyl Thiol Ester Dehydrase involved in a cyclic pathway [1] specifically the conversion of β-hydroxy acyl-ACP to Enol acyl-ACP as part of the fatty acid biosynthetic pathway [2].
 +
</p>
 +
          </div> 
</div>
</div>
 +
              <div class="leftbar">
                   <div class="block1">
                   <div class="block1">
                     <a href="https://2013.igem.org/Team:Manchester/Modellingtest">MODELLING</a>
                     <a href="https://2013.igem.org/Team:Manchester/Modellingtest">MODELLING</a>
Line 189: Line 396:
                   <div class="block3">
                   <div class="block3">
                     <a href="https://2013.igem.org/Team:Manchester/parametertest">PARAMETER ESTIMATION</a>
                     <a href="https://2013.igem.org/Team:Manchester/parametertest">PARAMETER ESTIMATION</a>
 +
                  </div>
 +
 +
                  <div class="block5">
 +
                    <a href="https://2013.igem.org/Team:Manchester/fabAmodeltest">fabA PROTEIN MODEL</a>
 +
                  </div>
 +
 +
                  <div class="block6">
 +
                    <a href="https://2013.igem.org/Team:Manchester/popdynamictest">POPULATION DYNAMICS</a>
                   </div>
                   </div>
                   <div class="block4">
                   <div class="block4">
                     <a href="https://2013.igem.org/Team:Manchester/collabtest">MODELLING COLLABORATION</a>
                     <a href="https://2013.igem.org/Team:Manchester/collabtest">MODELLING COLLABORATION</a>
 +
                  </div>               
 +
              </div>
 +
           
 +
              <!--<div class="rightbar">
 +
                  <div class="question1">
 +
                    <a href="#Q1">Introduction</a>
 +
                  </div>
 +
 +
                  <div class="question2">
 +
                    <a href="#Q2">Installing GROMACS</a>
 +
                  </div>
 +
                   
 +
                  <div class="question3">
 +
                    <a href="#Q3">Behind the scenes of a Molecular Dynamics Simulation</a>
 +
                  </div>
 +
 +
                  <div class="question4">
 +
                    <a href="#Q4">Getting a working GROMACS simulation for FabA</a>
 +
                  </div>
 +
 +
                  <div class="question5">
 +
                    <a href="#Q5">To His-tag or not to His-tag</a>
                   </div>
                   </div>
 +
            </div>-->       
</div>
</div>
</body>
</body>
</html>
</html>

Latest revision as of 14:48, 29 September 2013

page

Top

Safety

Introduction
Since the model of the fatty acid biosynthesis pathways highlighted several key enzymes that could be altered to produce palm oil, including Delta9, Delta12 and FabA, which agrees with previous publications on these enzymes. Delta9 and Delta12 are involved in linear pathways, meaning that there are obvious reactants and products, so the overexpression of them can be measured directly via LC-MS and other techniques. However, FabA is a β-Hydroxydecanoyl Thiol Ester Dehydrase involved in a cyclic pathway [1] specifically the conversion of β-hydroxy acyl-ACP to Enol acyl-ACP as part of the fatty acid biosynthetic pathway [2].

MODELLING
FATTY ACID PRODUCTION
PARAMETER ESTIMATION
fabA PROTEIN MODEL
POPULATION DYNAMICS
MODELLING COLLABORATION

Retrieved from "http://2013.igem.org/Team:Manchester/fattytest"