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Team:DTU-Denmark/Notebook/29 June 2013
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New PCR with x7-polymerase. | New PCR with x7-polymerase. | ||
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Kristian | Kristian | ||
Latest revision as of 18:08, 30 September 2013
29 June 2013
Contents |
208 lab
Main purposes today
New PCR with x7-polymerase.
Who was in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates.
- In tube 1+2, 5+6 where pZA21.
- In 9+10 GFP SF TAT
- In 3+4, 7+8 RFP
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.
Results
None of the PCRs worked.
Conclusion from today
New PCR programs need to be tested
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