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Team:DTU-Denmark/Notebook/29 June 2013
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=208 lab= | =208 lab= | ||
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== Main purposes today == | == Main purposes today == | ||
| + | <hr/> | ||
New PCR with x7-polymerase. | New PCR with x7-polymerase. | ||
| - | + | ==Who was in the lab== | |
| - | == | + | <hr/> |
Kristian | Kristian | ||
==Procedure== | ==Procedure== | ||
| + | <hr/> | ||
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates. | ||
| - | In tube 1+2, 5+6 where pZA21. | + | *In tube 1+2, 5+6 where pZA21. |
| - | In 9+10 GFP SF TAT | + | *In 9+10 GFP SF TAT |
| - | In 3+4, 7+8 RFP | + | *In 3+4, 7+8 RFP |
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. | First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. | ||
Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. | Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. | ||
Last program was 69°C annealing and 1:00 extension time with tube: 9+10. | Last program was 69°C annealing and 1:00 extension time with tube: 9+10. | ||
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==Results== | ==Results== | ||
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None of the PCRs worked. | None of the PCRs worked. | ||
| - | |||
== Conclusion from today == | == Conclusion from today == | ||
| - | + | <hr/> | |
| + | New PCR programs need to be tested | ||
| + | |||
| + | Navigate to the [[Team:DTU-Denmark/Notebook/28_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/30_June_2013|Next]] Entry | ||
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| + | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 18:08, 30 September 2013
29 June 2013
Contents |
208 lab
Main purposes today
New PCR with x7-polymerase.
Who was in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates.
- In tube 1+2, 5+6 where pZA21.
- In 9+10 GFP SF TAT
- In 3+4, 7+8 RFP
First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.
Results
None of the PCRs worked.
Conclusion from today
New PCR programs need to be tested
Navigate to the Previous or the Next Entry
